[CAS NO. 1185329-96-7] IXA4
PRODUCTS SPECIFICATIONS [1185329-96-7]
DESCRIPTION [1185329-96-7]
Overview
| MDL | - |
|---|---|
| Molecular Weight | 436.50 |
| Molecular Formula | C24H28N4O4 |
| SMILES | O=C(N(C)CCOC1=CC=C(C)C=C1)CN2N=CC(NC(CCOC3=CC=CC=C3)=O)=C2 |
Summary
IXA4 is a highly selective, non-toxic IRE1/XBP1s activator. IXA4 activates IRE1/XBP1s signaling without globally activating the unfolded protein response (UPR) or other stress-responsive signaling pathways (e.g., the heat shock response or oxidative stress response). IXA4 reduces secretion of APP through IRE1 activation [1] .
In Vitro
IXA4 (10 μM; 4 hours) selectively upregulates XBP1s mRNA, relative to genes regulated by ATF6 (e.g., BiP) or PERK (e.g., CHOP), in other cell lines including Huh7 and SHSY5Y cells
[1]
.
IXA4 (10μM; 18 hours) reduces Aβ levels 50% in conditioned media prepared on CHO7PA2 cells expressing the V717F APP (APPV717F) mutant
[1]
.
IXA4 rescues mitochondrial defects in SH-SY5Y cells expressing disease-relevant APP mutants. IXA4 (10μM; 4 hours) promotes adaptive IRE1/XBP1s signaling, but not RIDD, following 4 hrs of treatment in HEK293T cells
[1]
.
IXA4 also promotes selective transcriptional remodeling of ER proteostasis pathways, relative to cytosolic or mitochondrial pathways
[1]
.
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Appearance
Solid
Shipping
Room temperature in continental US; may vary elsewhere.
Storage
| Powder | -20°C | 3 years |
|---|---|---|
| 4°C | 2 years | |
| In solvent | -80°C | 6 months |
| -20°C | 1 month |
Solvent & Solubility
DMSO : 100 mg/mL ( 229.10 mM ; Need ultrasonic)
Stock Solutions
| Concentration Solvent Mass | 1 mg | 5 mg | 10 mg |
|---|
| 1 mM | 2.2910 mL | 11.4548 mL | 22.9095 mL |
| 5 mM | 0.4582 mL | 2.2910 mL | 4.5819 mL |
| 10 mM | 0.2291 mL | 1.1455 mL | 2.2910 mL |
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Add each solvent one by one: 10% DMSO >> 90% corn oil
Solubility: ≥ 2.5 mg/mL (5.73 mM); Clear solution