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[CAS NO. 1201652-50-7] Retro-2
| Ships within | Stock | Price | Qty | Total |
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| Catalog: | HY-122571 |
| Brand: | MCE |
| CAS: | 1201652-50-7 |
Overview
| MDL | - |
|---|---|
| Molecular Weight | 320.41 |
| Molecular Formula | C19H16N2OS |
| SMILES | O=C(NC1=CC=CC=C1)C2=CC=CC=C2/N=C/C3=CC=C(C)S3.[(E)] |
Summary
IC50 & Target
EC50: 12.2 µM (Ebolavirus) [3]
In Vitro
Retro-2 (1 μM; 1-4 hours) induces autophagy in GFP-LC3-expressing HeLa cells and promoted the dramatic cytoplasmic accumulation of large autophagosomes
[2]
.
Retro-2 (1 μM; 0.5-4 hours) treatment hows an increase over time of the abundance of LC3-II protein in cells
[2]
.
Retro-2 impairs the trafficking between autophagosomes and lysosomes. Retro-2 abolishes autolysosomes formation
[2]
.
Retro-2 (20 μM; pretreatment for 30 min) inhibits HeLa cell intoxication by ricin, Shiga-like toxins 1 (Stx1) and 2 (Stx2)
[1]
.
Retro-2 blocked invasion of cells by the intracellular parasite
Leishmania
, as well as replication of non-enveloped viruses, including polyoma-, papilloma-, and adeno-associated viruses
[3]
.
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Cell Autophagy Assay [2]
| Cell Line: | HeLa cells |
| Concentration: | 1 μM |
| Incubation Time: | 1 hour, 2 hours, 4 hours |
| Result: | Resulted in a large significant increase in the number of small and large vesicles. |
Western Blot Analysis [2]
| Cell Line: | HeLa cells |
| Concentration: | 1 μM |
| Incubation Time: | 0.5 hour, 2 hours, 4 hours |
| Result: | Showed an increase over time of the abundance of LC3-II protein in cells. |
In Vivo
Retro-2 (2-200 mg/kg; i.p; daily; for 21 days) treatment clearly protects from lethal nasal exposure to ricin in mice. 200 mg/kg of Retro-2 fully protects mice against ricin challenge [1] .
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
| Animal Model: | Female Balb/c mice (Pathogen-free 6-week-old) injected with ricin (2 μg/kg) [1] |
| Dosage: | 2 mg/kg, 10 mg/kg, 20 mg/kg, 200 mg/kg |
| Administration: | Intraperitoneal injection; daily; for 21 days |
| Result: | Protected mice against ricin challenge. |
Appearance
Solid
Shipping
Room temperature in continental US; may vary elsewhere.
Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
DMSO : 250 mg/mL ( 780.25 mM ; Need ultrasonic)
Stock Solutions
| Concentration Solvent Mass | 1 mg | 5 mg | 10 mg |
|---|
| 1 mM | 3.1210 mL | 15.6050 mL | 31.2100 mL |
| 5 mM | 0.6242 mL | 3.1210 mL | 6.2420 mL |
| 10 mM | 0.3121 mL | 1.5605 mL | 3.1210 mL |
References
- [1] Bahne Stechmann, et al. Inhibition of retrograde transport protects mice from lethal ricin challenge. Cell. 2010 Apr 16;141(2):231-42.
- [2] Valérie Nicolas, et al. Small Trafficking Inhibitor Retro-2 Disrupts the Microtubule-Dependent Trafficking of Autophagic Vacuoles. Front Cell Dev Biol. 2020 Jun 18;8:464.
- [3] Olena Shtanko, et al. Retro-2 and its dihydroquinazolinone derivatives inhibit filovirus infection. Antiviral Res. 2018 Jan;149:154-163.
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