[CAS NO. 122111-03-9] Gemcitabinehydrochloride

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PRODUCTS SPECIFICATIONS [122111-03-9]

Catalog

HY-B0003

Brand

MCE

CAS

122111-03-9

DESCRIPTION [122111-03-9]

Overview

MDL	MFCD01735988
Molecular Weight	299.66
Molecular Formula	C9H12ClF2N3O4
SMILES	O[C@H]1C(F)(F)[C@H](N2C(N=C(N)C=C2)=O)O[C@@H]1CO.Cl

For research use only. We do not sell to patients.

80 Publications Citing Use of MCE

Summary

Gemcitabine Hydrochloride (LY 188011 Hydrochloride) is a pyrimidine nucleoside analog antimetabolite and an antineoplastic agent. Gemcitabine Hydrochloride inhibits DNA synthesis and repair, resulting in autophagy and apoptosis ^[1] ^[2] .

IC50 & Target

DNA synthesis ^[1]

In Vitro

Gemcitabine Hydrochloride (purchased from MedChem Express, 0.003-1 μM; 3 days) kills both mouse and human senescent cells effectively and potently ^[4] .
Gemcitabine Hydrochloride inhibits the growth of BxPC-3, Mia Paca-2, PANC-1, PL-45 and AsPC-1 cells with IC ₅₀ s of 37.6, 42.9, 92.7, 89.3 and 131.4 nM, respectively ^[1] .

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay ^[4]

Cell Line:	Non-senescent and replication-induced senescent new born dermal fibroblasts (NBFs)
Concentration:	0.003, 0.01, 0.03, 0.1, 0.3, 1 μM
Incubation Time:	3 days
Result:	Killed replication-induced senescent NBFs for 3 days with 11.0% cell viability.

In Vivo

Gemcitabine Hydrochloride can be administered via endotracheal spray in rats without marked toxicity with a maximum tolerated dose of 4 mg/kg once a week for 9 weeks. The toxicity of Gemcitabine is lower via lung than oral administration at dosages of 2, 4, and 6 mg/kg ^[2] .
Treatment of the LSL-Kras ^G12D/+ ; LSL-Trp53 ^R172H ; Pdx-1-Cre mice with either Gemcitabine (50 mg/kg, i.p.) or the combination DMAPT/Gemcitabine Hydrochloride significantly increases the median survival time by more than 30 days compared to the placebo group (254.5 or 255 days vs. 217.5 days, respectively) ^[3] .

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial

Appearance

Solid

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture and light

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility

In Vitro:

H ₂ O : 33.33 mg/mL ( 111.23 mM ; Need ultrasonic)

DMSO : 25 mg/mL ( 83.43 mM ; ultrasonic and warming and heat to 60°C)

DMF : 2.5 mg/mL ( 8.34 mM ; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.3371 mL	16.6856 mL	33.3712 mL
5 mM	0.6674 mL	3.3371 mL	6.6742 mL
10 mM	0.3337 mL	1.6686 mL	3.3371 mL

* Please refer to the solubility information to select the appropriate solvent.

In Vivo:

1.

Add each solvent one by one: PBS

Solubility: 60 mg/mL (200.23 mM); Clear solution; Need ultrasonic
2.

Add each solvent one by one: 10% DMSO >> 40% PEG300 >> 5% Tween-80 >> 45% saline

Solubility: ≥ 2.08 mg/mL (6.94 mM); Clear solution
3.

Add each solvent one by one: 10% DMSO >> 90% (20% SBE-β-CD in saline)

Solubility: ≥ 2.08 mg/mL (6.94 mM); Clear solution
4.

Add each solvent one by one: 10% DMSO >> 90% corn oil

Solubility: ≥ 2.08 mg/mL (6.94 mM); Clear solution

* All of the co-solvents are available by MCE.

References

Synonyms

Cytidine, 2′-deoxy-2′,2′-difluoro-, hydrochloride (1:1)

Cytidine, 2′-deoxy-2′,2′-difluoro-, monohydrochloride

LY 188011 hydrochloride

Gemcitabine hydrochloride

Gemzar

Gemsar

Gemcitera

GEMORAL

2′-Deoxy-2′,2′-difluorocytidine monohydrochloride