[CAS NO. 126005-94-5] 28-Deoxonimbolide

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PRODUCTS SPECIFICATIONS [126005-94-5]

Catalog

HY-N1710

Brand

MCE

CAS

126005-94-5

DESCRIPTION [126005-94-5]

Overview

MDL	-
Molecular Weight	452.54
Molecular Formula	C27H32O6
SMILES	O=C(OC)C[C@@H]([C@]1(C)C2=C(C)[C@H](C3=COC=C3)C[C@@]2([H])O[C@]1([H])[C@@](OC4)([H])[C@@]5([H])[C@@]4(C)C=C6)[C@@]5(C)C6=O

For research use only. We do not sell to patients.

Summary

28-Deoxonimbolide is a nimbin (HY-N3187) type limonoid, that can be isolated from Azadirachta indica seed extracts. 28-Deoxonimbolide shows anticancer activity. 28-Deoxonimbolide induces apoptotic cell death in HL60 cells via both the mitochondrial- and the death receptor-mediated pathways ^[1] .

IC50 & Target

Caspase-8

Caspase-9

Caspase-3

In Vitro

28-Deoxonimbolide (1-100 μM, 48 h) exhibits potent cytotoxic activity against HL60 (leukemia), A549 (lung), AZ521 (stomach), SK-BR-3 (breast), CRL1579 (melanoma), and RPMI1788 (normal lymphocyte) cells ^[1] .
28-Deoxonimbolide (30 μM, 0-24 h) induces apoptosis in HL60 cells ^[1] .
28-Deoxonimbolide (30 μM, 0-24 h) increases the levels of cleaved caspases-8, -9, and -3 ^[1] .

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Cytotoxicity Assay ^[1]

Cell Line:	HL60, A549, AZ521, SK-BR-3, CRL1579 cells, RPMI1788
Concentration:	1-100 μM
Incubation Time:	48 h
Result:	Exhibited potent cytotoxic activity against HL60 (leukemia), A549 (lung), AZ521 (stomach), SK-BR-3 (breast), and CRL1579 (melanoma) cells, with IC ₅₀ values of 2.7 μM, 9.3 μM, 2.4 μM, 1.7 μM, 14.2 μM, and 2.7 μM, respectively.

Apoptosis Analysis ^[1]

Cell Line:	HL60 cells
Concentration:	30 μM
Incubation Time:	8 and 24 h
Result:	Induced early apoptosis in HL60 cells. The ratio of early apoptotic cells was increased after 8 h (10.9% vs 6.1% of negative control) and 24 h (18.7% vs 3.5% of negative control), and that of late apoptotic cells (upper right) was increased after 24 h (28.1% vs 2.9% of negative control).

Western Blot Analysis ^[1]

Cell Line:	HL60 cells
Concentration:	30 μM
Incubation Time:	8 and 24 h
Result:	Diminished the levels of procaspases-8, -9, and -3, and then increased the levels of cleaved caspases-8, -9, and -3.

Source

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Room temperature in continental US; may vary elsewhere.

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Please store the product under the recommended conditions in the Certificate of Analysis.

References

[1] Kikuchi T, et al. Cytotoxic and apoptosis-inducing activities of limonoids from the seeds of Azadirachta indica (neem). J Nat Prod. 2011 Apr 25;74(4):866-70.

Synonyms

2H,5H-Cyclopenta[b]furo[2′,3′,4′:4,5]naphtho[2,3-d]furan-6-acetic acid, 8-(3-furanyl)-2a,5a,6,6a,8,9,9a,10a,10b,10c-decahydro-2a,5a,6a,7-tetramethyl-5-oxo-, methyl ester, (2aR,5aR,6S,6aR,8R,9aR,10aS,10bR,10cS)-

2H,5H-Cyclopenta[d′]naphtho[1,8-bc:2,3-b′]difuran-6-acetic acid, 8-(3-furanyl)-2a,5a,6,6a,8,9,9a,10a,10b,10c-decahydro-2a,5a,6a,7-tetramethyl-5-oxo-, methyl ester, [2aR-(2aα,5aα,6α,6aα,8β,9aβ,10aα,10bα,10cβ)]-

2H,5H-Cyclopenta[d′]naphtho[1,8-bc:2,3-b′]difuran-6-acetic acid, 8-(3-furanyl)-2a,5a,6,6a,8,9,9a,10a,10b,10c-decahydro-2a,5a,6a,7-tetramethyl-5-oxo-, methyl ester, (2aR,5aR,6S,6aR,8R,9aR,10aS,10bR,10cS)-

28-Deoxonimbolide