[CAS NO. 1290541-46-6] RAD51 Inhibitor B02
PRODUCTS SPECIFICATIONS [1290541-46-6]
DESCRIPTION [1290541-46-6]
Overview
| MDL | MFCD03294274 |
|---|---|
| Molecular Weight | 339.39 |
| Molecular Formula | C22H17N3O |
| SMILES | O=C1N(CC2=CC=CC=C2)C(/C=C/C3=CC=CN=C3)=NC4=C1C=CC=C4 |
4 Publications Citing Use of MCE
Summary
RAD51 Inhibitor B02 (B02) is an inhibitor of human RAD51 with an IC 50 of 27.4 μM.
IC50 & Target
IC50: 27.4 μM (hRAD51) [1]
In Vitro
RAD51 Inhibitor B02 specifically inhibits human RAD51 (IC 50 =27.4 μM), but not its E. coli homologue RecA (IC 50 >250 μM) [1] . The combination of B02 with cisplatin has the strongest killing effect on the human breast cancer cells MDA-MB-231 [2] .
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
In Vivo
B02 significantly enhances the therapeutic effect of cisplatin on tumor cells in vivo . B02 is tolerated by mice at doses up to 50 mg/kg without obvious body weight loss. No inhibition of tumor growth is observed on mice solely treated by B02. Mice treated with 4 mg/kg cisplatin, however, shows a 33% inhibition of tumor growth. Finally, mice treated with 50 mg/kg B02 and 4 mg/kg cisplatin shows a 66% inhibition of tumor growth [2] .
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Appearance
Solid
Shipping
Room temperature in continental US; may vary elsewhere.
Storage
| Powder | -20°C | 3 years |
|---|---|---|
| 4°C | 2 years | |
| In solvent | -80°C | 6 months |
| -20°C | 1 month |
Solvent & Solubility
DMSO : ≥ 37 mg/mL ( 109.02 mM )
* "≥" means soluble, but saturation unknown.
Stock Solutions
| Concentration Solvent Mass | 1 mg | 5 mg | 10 mg |
|---|
| 1 mM | 2.9465 mL | 14.7323 mL | 29.4646 mL |
| 5 mM | 0.5893 mL | 2.9465 mL | 5.8929 mL |
| 10 mM | 0.2946 mL | 1.4732 mL | 2.9465 mL |
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1.
Add each solvent one by one: 20% DMSO >> 20% Cremophor EL >> 60% Saline
Solubility: 10 mg/mL (29.46 mM); Clear solution; Need ultrasonic
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2.
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3.
Add each solvent one by one: 10% DMSO >> 90% corn oil
Solubility: ≥ 2 mg/mL (5.89 mM); Clear solution
References
- [1] Huang F, et al. Identification of specific inhibitors of human RAD51 recombinase using high-throughput screening. ACS Chem Biol. 2011 Jun 17;6(6):628-35.
- [2] Huang F, et al. A small molecule inhibitor of human RAD51 potentiates breast cancer cell killing by therapeutic agents in mouse xenografts. PLoS One. 2014 Jun 27;9(6):e100993.