[CAS NO. 1358-76-5] Koumine

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PRODUCTS SPECIFICATIONS [1358-76-5]

Catalog

HY-N1440

Brand

MCE

CAS

1358-76-5

DESCRIPTION [1358-76-5]

Overview

MDL	MFCD11111448
Molecular Weight	306.40
Molecular Formula	C20H22N2O
SMILES	C=C[C@]12[C@]3([H])C[C@@H]4C5=NC6=CC=CC=C6[C@@]51C[C@H](N(C)C2)[C@@]3([H])CO4

For research use only. We do not sell to patients.

Summary

Koumine is an alkaloid separated from Gelsemium elegans , shows potent anti-tumor activity. Koumine up-regulates the Bax/Bcl-2 ratio and caspase-3 expression in human breast cancer cells ^[1] . Koumine has anxiolytic, antistress, antipsoriatic, and analgesic activities ^[3] , protects against the development of arthritis in Rheumatoid arthritis (RA) animal models ^[2] .

In Vitro

Koumine (0.5, 1 and 2 mg/mL) dose- and time-dependently inhibits the proliferation of MCF-7 cells, with an IC ₅₀ of 124 µg/mL at 72 h. Koumine induces apoptosis, causes cell cycle arrest at G2/M phase ^[1] .
Koumine (0.5, 1 and 2 mg/mL) up-regulates the Bax/Bcl-2 ratio and caspase-3 expression in a dose-dependent manner in MCF-7 Cells ^[1] .
Koumine (25, 50, 100, and 200 μM) decreases the protein and mRNA levels of microglia M1 polarization factors in LPS-induced BV2 cells ^[3] .

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Koumine is less toxic, with the median lethal dose (LD ₅₀ ) of 300.0 mg/kg on Wistar rats. Koumine (0.6, 3, or 15 mg/kg/per, p.o.) exhibits antirheumatic properties in rats with adjuvant-induced arthritis (AIA) and collagen-induced arthritis (CIA) ^[2] .
Koumine inhibits the increase in cytokines in joint tissue and TNF-α level in serum at 15 mg/kg, and suppresses the increase in the serum level of IL-1β at 3 and 15 mg/kg ^[2] .
Koumine (0.28, 7 mg/kg, s.c.) significantly reduces neuropathic pain after nerve injury. Koumine suppresses the increased Iba-1 protein level ^[3] .

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Appearance

Solid

Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture and light

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility

In Vitro:

DMSO : 12.5 mg/mL ( 40.80 mM ; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.2637 mL	16.3185 mL	32.6371 mL
5 mM	0.6527 mL	3.2637 mL	6.5274 mL
10 mM	0.3264 mL	1.6319 mL	3.2637 mL

* Please refer to the solubility information to select the appropriate solvent.

In Vivo:

1.

Add each solvent one by one: 10% DMSO >> 40% PEG300 >> 5% Tween-80 >> 45% saline

Solubility: ≥ 2.08 mg/mL (6.79 mM); Clear solution
2.

Add each solvent one by one: 10% DMSO >> 90% (20% SBE-β-CD in saline)

Solubility: ≥ 2.08 mg/mL (6.79 mM); Clear solution
3.

Add each solvent one by one: 10% DMSO >> 90% corn oil

Solubility: ≥ 2.08 mg/mL (6.79 mM); Clear solution

* All of the co-solvents are available by MCE.

References

Synonyms

6,4-(Epoxymethano)-3,11b-methano-11bH-pyrido[4,3-c]carbazole, 11c-ethenyl-1,2,3,4,4a,5,6,11c-octahydro-2-methyl-, (3S,4S,4aR,6R,11bS,11cS)-

Koumine

7,20(2H,19H)-Cyclovobasan, 1,2,18,19-tetradehydro-3,17-epoxy-, (3R,7α,20α)-

6,4-(Epoxymethano)-3,11b-methano-11bH-pyrido[4,3-c]carbazole, 7,20(2H,19H)-cyclovobasan deriv.

(3S,4S,4aR,6R,11bS,11cS)-11c-Ethenyl-1,2,3,4,4a,5,6,11c-octahydro-2-methyl-6,4-(epoxymethano)-3,11b-methano-11bH-pyrido[4,3-c]carbazole