[CAS NO. 1393922-01-4] Millepachine
PRODUCTS SPECIFICATIONS [1393922-01-4]
DESCRIPTION [1393922-01-4]
Overview
| MDL | - |
|---|---|
| Molecular Weight | 350.41 |
| Molecular Formula | C22H22O4 |
| SMILES | O=C(C1=C2C(C=CC(C)(C)O2)=C(OC)C=C1)/C=C/C3=CC=C(OC)C=C3 |
Summary
In Vitro
Millepachine (1.25-20 μM; 48 h) remarkably inhibits the proliferation of cisplatin-resistant A2780CP cells
[1]
.
Millepachine (2-8 μM; 24 or 48 h) induces G2/M arrest and apoptosis cisplatin-sensitive A2780S and cisplatin-resistant A2780CP cells
[1]
.
Millepachine (2-8 μM; 24 h) decreases topoisomerase II levels in A2780S and A2780CP cells
[1]
.
Millepachine (2-8 μM; 24 h) inhibits ATP-binding cassette transporter activity in A2780CP cells
[1]
.
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Cell Proliferation Assay [1]
| Cell Line: | A2780CP cells |
| Concentration: | 0, 1.25, 2.5, 5, 10, 20 μM |
| Incubation Time: | 48 hours |
| Result: | Inhibited the cells proliferation with an IC 50 of 4 μM. |
Cell Cycle Analysis [1]
| Cell Line: | A2780S and A2780CP cells |
| Concentration: | 2, 4, 8 μM |
| Incubation Time: | 24 or 48 hours |
| Result: |
Induced significant G2/M arrest both in both cells.
The percentage of cells in the G2/M fraction increased from 15.99% in vehicle cells to 24.93%, 60.67%, and 77.31% at dose of 2, 4, and 8 μM in A2780S cells, respectively. |
Apoptosis Analysis [1]
| Cell Line: | A2780S and A2780CP cells |
| Concentration: | 2, 4, 8 μM |
| Incubation Time: | 24 or 48 hours |
| Result: |
The percentage of apoptotic cells increased from 1.49% in vehicle cells to 10.98%, 20.60%, and 39.43% at dose of 2, 4, and 8 μM in A2780S cells, respectively.
The percentage of apoptotic cells increased from 0.87% to 10.97%, 25.28%, and 37.59% in A2780CP cells, respectively. |
Western Blot Analysis [1]
| Cell Line: | A2780S and A2780CP cells |
| Concentration: | 0, 2, 4, 8 μM |
| Incubation Time: | 24 hours |
| Result: | Decreased the levels of topoisomerase II (TOPO II) in both cells. |
In Vivo
Millepachine (20 mg/kg; i.v. every two days for 14 day) inhibits tumor growth in mice
[1]
.
Millepachine (20 mg/kg; i.v. every two days for 14 day) does not induce acquired drug resistance in an excised A2780S xenograft model
[1]
.
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
| Animal Model: | Female BALB/c nude mice (6 weeks) are injected A2780S or A2780CP cells [1] |
| Dosage: | 20 mg/kg |
| Administration: | I.v. every two days for 2-14 days |
| Result: |
Reduced tumor volume and tumor weight with the inhibitory rate of 73.21% and 65.58% in A2780S (after seven times injection) and A2780CP (after six times injection) xenograft model, respectively.
With low toxicity in vivo. |
Appearance
Solid
Shipping
Room temperature in continental US; may vary elsewhere.
Storage
| Powder | -20°C | 3 years |
|---|---|---|
| 4°C | 2 years | |
| In solvent | -80°C | 6 months |
| -20°C | 1 month |
Solvent & Solubility
DMSO : 100 mg/mL ( 285.38 mM ; Need ultrasonic)
Stock Solutions
| Concentration Solvent Mass | 1 mg | 5 mg | 10 mg |
|---|
| 1 mM | 2.8538 mL | 14.2690 mL | 28.5380 mL |
| 5 mM | 0.5708 mL | 2.8538 mL | 5.7076 mL |
| 10 mM | 0.2854 mL | 1.4269 mL | 2.8538 mL |
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1.
-
2.
Add each solvent one by one: 10% DMSO >> 90% corn oil
Solubility: ≥ 2.5 mg/mL (7.13 mM); Clear solution