[CAS NO. 1419320-73-2] Pitstop2
PRODUCTS SPECIFICATIONS [1419320-73-2]
DESCRIPTION [1419320-73-2]
Overview
| MDL | - |
|---|---|
| Molecular Weight | 473.36 |
| Molecular Formula | C20H13BrN2O3S2 |
| SMILES | O=S(C1=C2C=CC=CC2=CC=C1)(NC(S/C3=C\C4=CC=C(Br)C=C4)=NC3=O)=O.[(Z)] |
6 Publications Citing Use of MCE
Summary
In Vitro
Pitstop 2 (20-40 μM; 30 min) inhibits the endocytosis of transferrin in J774A.1 macrophages and does not compromise cell viability
[1]
.
Pitstop 2 (20-40 μM; 30 min) does not affect the internalization of cholera toxin B in J774A.1 macrophages
[1]
.
Pitstop 2 (0.001-100 μM; 6 h) inhibits the mitotic spindle and impairs mitotic progression in HeLa cells
[2]
.
Pitstop 2 (1-30 μM; 24 h) induces apoptosis and inhibits cell growth in dividing cancer cells
[2]
.
Pitstop 2 (1-30 μM; 48 h) does not affect the growth and viability of non-tumourigenic NIH3T3 fibroblasts
[2]
.
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Cell Viability Assay [2]
| Cell Line: | HeLa cells |
| Concentration: | 1, 3, 10, 30 μM |
| Incubation Time: | 24 hours |
| Result: | Reduced the total number of viable HeLa cells markedly in a dose-dependent manner. |
Appearance
Solid
Shipping
Room temperature in continental US; may vary elsewhere.
Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
DMSO : 62.5 mg/mL ( 132.03 mM ; Need ultrasonic)
Stock Solutions
| Concentration Solvent Mass | 1 mg | 5 mg | 10 mg |
|---|
| 1 mM | 2.1126 mL | 10.5628 mL | 21.1256 mL |
| 5 mM | 0.4225 mL | 2.1126 mL | 4.2251 mL |
| 10 mM | 0.2113 mL | 1.0563 mL | 2.1126 mL |
References
- [1] Kumar GA, et, al. Leishmania donovani Internalizes into Host Cells via Caveolin-mediated Endocytosis. Sci Rep. 2019 Sep 2; 9(1): 12636.
- [2] Smith CM, et, al. Inhibition of clathrin by pitstop 2 activates the spindle assembly checkpoint and induces cell death in dividing HeLa cancer cells. Mol Cancer. 2013 Jan 17; 12:4.