[CAS NO. 1426944-49-1] HBX 19818

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PRODUCTS SPECIFICATIONS [1426944-49-1]

Catalog

HY-17540

Brand

MCE

CAS

1426944-49-1

DESCRIPTION [1426944-49-1]

Overview

MDL	MFCD28167744
Molecular Weight	421.96
Molecular Formula	C25H28ClN3O
SMILES	CN(CCCNC(C1=CC=C(N=C(CCCC2)C2=C3Cl)C3=C1)=O)CC4=CC=CC=C4

For research use only. We do not sell to patients.

11 Publications Citing Use of MCE

Summary

HBX 19818 is a specific inhibitor of ubiquitin-specific protease 7 (USP7) , with an IC ₅₀ of 28.1 μM.

IC50 & Target

IC50: 28.1 μM (USP7) ^[1]

In Vitro

HBX 19818 is an inhibitor of USP7, with an IC ₅₀ of 28.1 μM. HBX 19818 shows no effects on USP8, USP5, USP10, CYLD, UCH-L1, UCH-L3 or on SENP1, a SUMO protease, with IC ₅₀ s of > 200 μM. HBX 19818 selectively inhibits USP7 with IC ₅₀ of ∼6 μM in in human cancer cells. In addition, HBX 19818 (1.5, 4, 12, 36, or 100 μM) inhibits USP7 deubiquitination of polyubiquitinated p53. HBX 19818 (30 μM) also causes significantly higher levels of Mdm2 polyubiquitinated forms in USP7-overproducing HEK293 cells than those in DMSO-treated control cells. HBX 19818 inhibits HCT116 proliferation in a dose-dependent manner, with an IC ₅₀ of ∼2 μM ^[1] .

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Appearance

Solid

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

1M HCl : 100 mg/mL ( 236.99 mM ; ultrasonic and adjust pH to 1 with HCl)

DMSO : 20 mg/mL ( 47.40 mM ; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.3699 mL	11.8495 mL	23.6989 mL
5 mM	0.4740 mL	2.3699 mL	4.7398 mL
10 mM	0.2370 mL	1.1849 mL	2.3699 mL

* Please refer to the solubility information to select the appropriate solvent.

In Vivo:

1.

Add each solvent one by one: 10% DMSO >> 40% PEG300 >> 5% Tween-80 >> 45% saline

Solubility: ≥ 2 mg/mL (4.74 mM); Clear solution
2.

Add each solvent one by one: 10% DMSO >> 90% corn oil

Solubility: ≥ 2 mg/mL (4.74 mM); Clear solution

* All of the co-solvents are available by MCE.

References

[1] Reverdy C, et al. Discovery of specific inhibitors of human USP7/HAUSP deubiquitinating enzyme. Chem Biol. 2012 Apr 20;19(4):467-77.