[CAS NO. 1448846-35-2] LPd peroxida probe
PRODUCTS SPECIFICATIONS [1448846-35-2]
DESCRIPTION [1448846-35-2]
Overview
| MDL | - |
|---|---|
| Molecular Weight | 840.85 |
| Molecular Formula | C51H41N2O8P |
| SMILES | O=C1C2=CC=C3C4=CC=C5C(N(CCOCCOCCOCCOC)C(C6=CC=C(C4=C56)C7=C3C2=C(C(N1C8=CC=C(P(C9=CC=CC=C9)C%10=CC=CC=C%10)C=C8)=O)C=C7)=O)=O |
Summary
Liperfluo, a marker of ferroptosis , is a useful fluorescent probe for investigating the roles of lipid peroxidation in a variety of cell pathophysiologies. Liperfluo reduces lipid hydroperoxides to lipid alcohols and is used for imaging lipid hydroperoxides in living cells [1] [2] [3] .
In Vitro
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
1. Cells in the glass bottom dish are treated with 50 µM of H
2
O
2
in RPMI 1640 for 2 h at 37 °C. After removing H
2
O
2
, cells are washed with Hank’s balanced salt solution (HBSS) three times.
2. Cells are then treated with 10 µM of Liperfluo in HBSS for 30 min at 37 °C. After removing Liperfluo, cells are washed with HBSS three times.
3. Fluorescence images are obtained using a BZ-8000 fluorescence microscope from 3 separate dishes for each treatment
[1]
.
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Shipping
Room temperature in continental US; may vary elsewhere.
Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
References
- [1] Kazuo Tomita, et al. MiR-7-5p Is Involved in Ferroptosis Signaling and Radioresistance Thru the Generation of ROS in Radioresistant HeLa and SAS Cell Lines. Int J Mol Sci. 2021 Aug 2;22(15):8300.
- [2] Elizabeth M Kenny, et al. Ferroptosis Contributes to Neuronal Death and Functional Outcome After Traumatic Brain Injury. Crit Care Med. 2019 Mar;47(3):410-418.
- [3] Kazunori Yamanaka, et al. A novel fluorescent probe with high sensitivity and selective detection of lipid hydroperoxides in cells. RSC Advances, 2012, 2, 7894–7900.