[CAS NO. 14930-96-2] CytochalasinB

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PRODUCTS SPECIFICATIONS [14930-96-2]

Catalog

HY-16928

Brand

MCE

CAS

14930-96-2

DESCRIPTION [14930-96-2]

Overview

MDL	-
Molecular Weight	479.61
Molecular Formula	C29H37NO5
SMILES	O=C1N[C@@H](CC2=CC=CC=C2)[C@@]3([H])[C@]14[C@](/C=C/C[C@H](C)CCC[C@@H](O)/C=C/C(O4)=O)([H])[C@H](O)C([C@H]3C)=C

For research use only. We do not sell to patients.

13 Publications Citing Use of MCE

Summary

Cytochalasin B is a cell-permeable mycotoxin binding to the barbed end of actin filaments, disrupting the formation of actin polymers , with K _d value of 1.4-2.2 nM for F-actin. Cytochalasin B blocks cell migration.

IC50 & Target

Kd: 2.2 nM (F-actin, with Mg ²⁺ ), 1.4 nM (F-actin, with Mg ²⁺ /K ⁺ ) ^[1]

In Vitro

Cytochalasin B is a cell-permeable mycotoxin binding to the barbed end of actin filaments, inhibits the enlongation and shortening of actin filaments, with K _d s of 2.2 nM and 1.4 nM for F-actin in the presence of MgCl ₂ (2 mM) or MgCl ₂ (2 mM) plus KCl, respectively ^[1] . Cytochalasin B (0.1-10 μM) shows inhibitory effect on multiple murine cancer cell lines, with IC ₅₀ s of 2.56 μM (M109c), 10.46 μM (B16BL6), 105.5 μM (P388/ADR), 51.9 μM (P388/S) and IC ₈₀ s of 12.23 μM (M109c), 44.86 μM (B16BL6), 188.4 μM (P388/ADR), 84.1 μM (P388/S) after treatment for 3 h, with IC ₅₀ s of 0.25 μM (M109c), 0.37 μM (B16F10), 0.87 μM (B16BL6), and IC ₈₀ s of 0.75 μM (M109c), 1.21 μM (B16F10), 10.41 μM (B16BL6) after treatment for 4 days ^[2] . Cytochalasin B (6 μM) increases the myofibrillar fragmentation index (MFI), which is attributed to the intensely breaking of myofibrillar proteins into short segments. Cytochalasin B also accelerates the disruption of actin filaments. In addition, Cytochalasin B accelerates the transformation from F-actin to G-actin, lowering the content of F-actin and significantly increasing G-actin bands during postmortem conditioning ^[3] .

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Cytochalasin B (10, 25, 50 mg/kg, i.p.) dose-dependently increases the life expectancy of Balb/c mice bearing with P388/ADR leukemias. Cytochalasin B at 50 mg/kg produces 10 % long-term survival in the multidrug resistant P388/ADR cohort, and 40 % long-term survival in the drug sensitive P388/S cohort ^[2] .

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial

NCT Number	Sponsor	Condition	Start Date	Phase
NCT03678558	Ibn Sina Hospital\|Banoon IVF Center	Infertility	September 25, 2018	Not Applicable
NCT03663634	Ibn Sina Hospital\|Banoon IVF Center	Infertility	September 16, 2018	Not Applicable

Appearance

Solid

Source

Mould

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 83.33 mg/mL ( 173.75 mM ; ultrasonic and warming and heat to 60°C)

Ethanol : 25 mg/mL ( 52.13 mM ; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.0850 mL	10.4251 mL	20.8503 mL
5 mM	0.4170 mL	2.0850 mL	4.1701 mL
10 mM	0.2085 mL	1.0425 mL	2.0850 mL

* Please refer to the solubility information to select the appropriate solvent.

In Vivo:

1.

Add each solvent one by one: 0.5% CMC-Na >> 0.5% Tween-80

Solubility: 5 mg/mL (10.43 mM); Suspened solution; Need ultrasonic
2.

Add each solvent one by one: 10% EtOH >> 40% PEG300 >> 5% Tween-80 >> 45% saline

Solubility: ≥ 2.5 mg/mL (5.21 mM); Clear solution
3.

Add each solvent one by one: 10% EtOH >> 90% (20% SBE-β-CD in saline)

Solubility: ≥ 2.5 mg/mL (5.21 mM); Clear solution
4.

Add each solvent one by one: 10% EtOH >> 90% corn oil

Solubility: ≥ 2.5 mg/mL (5.21 mM); Clear solution
5.

Add each solvent one by one: 10% DMSO >> 40% PEG300 >> 5% Tween-80 >> 45% saline

Solubility: ≥ 2.08 mg/mL (4.34 mM); Clear solution
6.

Add each solvent one by one: 10% DMSO >> 90% (20% SBE-β-CD in saline)

Solubility: ≥ 2.08 mg/mL (4.34 mM); Clear solution
7.

Add each solvent one by one: 10% DMSO >> 90% corn oil

Solubility: ≥ 2.08 mg/mL (4.34 mM); Clear solution

* All of the co-solvents are available by MCE.

References

Synonyms

2H-Oxacyclotetradecino[2,3-d]isoindole-2,18(5H)-dione, 6,7,8,9,10,12a,13,14,15,15a,16,17-dodecahydro-5,13-dihydroxy-9,15-dimethyl-14-methylene-16-(phenylmethyl)-, (3E,5R,9R,11E,12aS,13S,15S,15aS,16S,18aS)-

2H-Oxacyclotetradecino[2,3-d]isoindole-2,18(5H)-dione, 16-benzyl-6,7,8,9,10,12a,13,14,15,15a,16,17-dodecahydro-5,13-dihydroxy-9,15-dimethyl-14-methylene-, (E,E)-(5R,9R,12aS,13S,15S,15aS,16S,18aS)-

24-Oxa[14]cytochalasa-6(12),13,21-triene-1,23-dione, 7,20-dihydroxy-16-methyl-10-phenyl-, (7S,13E,16R,20R,21E)-

(3E,5R,9R,11E,12aS,13S,15S,15aS,16S,18aS)-6,7,8,9,10,12a,13,14,15,15a,16,17-Dodecahydro-5,13-dihydroxy-9,15-dimethyl-14-methylene-16-(phenylmethyl)-2H-oxacyclotetradecino[2,3-d]isoindole-2,18(5H)-dione

Cytochalasin B

Phomin

2H-Oxacyclotetradecino[2,3-d]isoindole-2,18(5H)-dione, 6,7,8,9,10,12a,13,14,15,15a,16,17-dodecahydro-5,13-dihydroxy-9,15-dimethyl-14-methylene-16-(phenylmethyl)-, [5R-(3E,5R*,9R*,11E,12aS*,13S*,15S*,15aS*,16S*,18aS*)]-

NSC 107658