[CAS NO. 151358-47-3] DIM-C-pPhOH

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PRODUCTS SPECIFICATIONS [151358-47-3]

Catalog

HY-112055

Brand

MCE

CAS

151358-47-3

DESCRIPTION [151358-47-3]

Overview

MDL	MFCD03723253
Molecular Weight	338.40
Molecular Formula	C23H18N2O
SMILES	OC1=CC=C(C(C2=CNC3=C2C=CC=C3)C4=CNC5=C4C=CC=C5)C=C1

For research use only. We do not sell to patients.

1 Publications Citing Use of MCE

[1]Redox Biol. 2021 Jan;38:101807.

Summary

DIM-C-pPhOH is a nuclear receptor 4A1 (NR4A1) antagonist. DIM-C-pPhOH inhibits cancer cell growth and mTOR signaling, induce apoptosis and cellular stress. DIM-C-pPhOH reduces cell proliferation with IC50 values of 13.6 μM and 13.0 μM for ACHN cells and 786-O cells, respectively ^[1] .

IC50 & Target

Nuclear receptor 4A1 (NR4A1) ^[1]

In Vitro

DIM-C-pPhOH (7.5-20 μM; 24 hours; ACHN and 786-O cells) treatment significantly decreases cell proliferation ^[1] .
DIM-C-pPhOH (20 μM; 24 hours; ACHN and 786-O cells) treatment induces Annexin V staining in ACHN and 786-O cells, confirming that DIM-C-pPhOH induce apoptosis, and also induces cleavage of caspases 7 and 8 ^[1] .
DIM-C-pPhOH (15-20 μM; 24 hours; ACHN and 786-O cells) treatment inhibits NR4A1-regulated expression of survivin, bcl-2 and EGFR in ACHN and 786-O cells. And also induces sestrin 2, activates AMPKα and inhibits activation of mTOR and downstream kinases ^[1] .
DIM-C-pPhOH decreases expression of β1-integrin protein and mRNA and β1-integrin-dependent responses in MCF7, MDA-MB-231, and SKBR3 cells and also inhibits migration of the latter two cell lines ^[2] .

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay ^[1]

Cell Line:	ACHN and 786-O cells
Concentration:	7.5 μM, 15 μM, 20 μM
Incubation Time:	24 hours
Result:	Significantly decreased cell proliferation.

Apoptosis Analysis ^[1]

Cell Line:	ACHN and 786-O cells
Concentration:	20 μM
Incubation Time:	24 hours
Result:	Induced apoptosis in ACHN and 786-O cells.

Western Blot Analysis ^[1]

Cell Line:	ACHN and 786-O cells
Concentration:	15 μM, 20 μM
Incubation Time:	24 hours
Result:	Inhibited NR4A1-regulated expression of survivin, bcl-2 and EGFR in ACHN and 786-O cells. And also induced sestrin 2, activated AMPKα and inhibited activation of mTOR and downstream kinases.

In Vivo

DIM-C-pPhOH (30 mg/kg; oral gavage; daily; for 50 days; male athymic nude mice) treatment results in a significant inhibition of tumor growth ^[1] .

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Male athymic nude mice (aged 6-7 weeks) injected with ACHN cells ^[1]
Dosage:	30 mg/kg/day
Administration:	Oral gavage; daily; for 50 days
Result:	Resulted in a significant inhibition of tumor growth.

Appearance

Solid

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 125 mg/mL ( 369.39 mM ; ultrasonic and warming and heat to 60°C)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.9551 mL	14.7754 mL	29.5508 mL
5 mM	0.5910 mL	2.9551 mL	5.9102 mL
10 mM	0.2955 mL	1.4775 mL	2.9551 mL

* Please refer to the solubility information to select the appropriate solvent.

In Vivo:

1.

Add each solvent one by one: 10% DMSO >> 40% PEG300 >> 5% Tween-80 >> 45% saline

Solubility: ≥ 2.08 mg/mL (6.15 mM); Clear solution
2.

Add each solvent one by one: 10% DMSO >> 90% (20% SBE-β-CD in saline)

Solubility: ≥ 2.08 mg/mL (6.15 mM); Clear solution
3.

Add each solvent one by one: 10% DMSO >> 90% corn oil

Solubility: ≥ 2.08 mg/mL (6.15 mM); Clear solution

* All of the co-solvents are available by MCE.