[CAS NO. 154822-57-8] Cimiside E
PRODUCTS SPECIFICATIONS [154822-57-8]
DESCRIPTION [154822-57-8]
Overview
| MDL | - |
|---|---|
| Molecular Weight | 602.80 |
| Molecular Formula | C35H54O8 |
| SMILES | C[C@]12[C@@]3([H])[C@@]4(CC[C@@]1([C@]5([H])[C@@]6(O[C@]([C@@H](O6)C(C)=C)([H])C[C@H]5C)[C@@H]2O)C)[C@@]7([C@@](C(C)([C@H](CC7)O[C@H]8[C@@H]([C@H]([C@@H](CO8)O)O)O)C)([H])CC3)C4 |
Summary
Cimiside E (25-Anhydrocimigenol xyloside) is a triterpene xyloside, Cimiside E possesses apoptotic action on gastric cancer cells, with an IC 50 value of 14.58 μM. Cimiside E induces cell cycle arrest at G2/M phase, and mediates apoptosis through the induction of the caspase cascade for both the extrinsic and intrinsic pathways [1] [2] .
IC50 & Target
|
Caspase 3 |
In Vitro
Cimiside E (30-90 μM; 24 h) arrest cell cycle and induces apoptosis in ASG cells
[1]
.
Cimiside E (30-90 μM; 12-48 h) has a strong cytotoxicity on AGS cells and shows anti-proliferative activity
[1]
.
Cimiside E (15-60 μM; 6-24 h) induces DNA fragment, (30-60; 1-6 h) activates expression of FasL at 3 h and Fas from 1 h in ASG cells
[1]
.
Cimiside E (30-90 μM; 3-24 h) mediates caspase cascade, by increasing Bax/Bcl-2 ratio and decreasing mutant type (mt) p53 and procaspase 3
[1]
.
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Western Blot Analysis [1]
| Cell Line: | Cimiside E. AGS cells |
| Concentration: | 30 μM, 60 μM, and 90 μM |
| Incubation Time: | 3 h, 6 h, 12 h, and 24 h |
| Result: |
Increased the ratio of Bax/Bcl-2 expression from 60 μM.
Decreased mutant type (mt) p53 levle from 12 h at 30 µM. Suppressed the protein level of procaspase 3 in a dose-dependent manner from 30 μM. |
Cell Proliferation Assay [1]
| Cell Line: | Cimiside E. AGS cells |
| Concentration: | 30 μM, 60 μM, and 90 μM |
| Incubation Time: | 12 h, 24 h, and 48 h |
| Result: | Inhibited ASG cells proliferation with IC 50 s of 28.7, 14.6 and 8.1 µM, respectively, for 30 μM, 60 μM, and 90 μM treatment. |
Cell Cycle Analysis [1]
| Cell Line: | Cimiside E. AGS cells |
| Concentration: | 30 μM, 60 μM, and 90 μM |
| Incubation Time: | 3 h, 6 h, and 24 h |
| Result: | Induced cell cycle arrest at S phase in a low concentration (30 μM), but arrested cell cycle at G2/M phase in higher concentration (60 μM and 90 μM). |
Shipping
Room temperature in continental US; may vary elsewhere.
Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
References
- [1] Guo LY, et al. Cimiside E arrests cell cycle and induces cell apoptosis in gastric cancer cells. Arch Pharm Res. 2009 Oct;32(10):1385-92.
- [2] Jamróz MK, et al. One new and six known triterpene xylosides from Cimicifuga racemosa: FT-IR, Raman and NMR studies and DFT calculations. Spectrochim Acta A Mol Biomol Spectrosc. 2012 Jul;93:10-8.