[CAS NO. 1601496-05-2] GSK-843

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PRODUCTS SPECIFICATIONS [1601496-05-2]

Catalog

HY-125402

Brand

MCE

CAS

1601496-05-2

DESCRIPTION [1601496-05-2]

Overview

MDL	-
Molecular Weight	377.49
Molecular Formula	C19H15N5S2
SMILES	NC1=NC=C(C2=CC(C)=NN2C)C3=C1C(C4=CC=C(SC=N5)C5=C4)=CS3

For research use only. We do not sell to patients.

2 Publications Citing Use of MCE

Summary

GSK-843 (GSK'843) is a receptor-interacting protein kinase 3 (RIP3 or RIPK3) inhibitor, which binds RIP3 kinase domain with an IC ₅₀ of 8.6 nM, and inhibits kinase activity with an IC ₅₀ of 6.5 nM. GSK-843 can be used for the research of inflammation ^[1] ^[2] .

IC50 & Target

IC50: 6.5 nM (RIP3) ^[1]

In Vitro

GSK-843 (3-10 μM; 18 h) induces apoptosis ^[1] .
GSK-843 (0.3-3 μM; 18 h) suppresses TNF-induced death and virus-induced cell necrosis ^[2] .

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay ^[1]

Cell Line:	SVEC, L929, 3T3SA and mouse embryo fibroblast (MEF)
Concentration:	3 and 10 μM
Incubation Time:	18 hours
Result:	Decreased the cell viability of SVEC, L929, 3T3SA and MEF cells, and induced caspase activation followed by apoptotic cell death.

Cell Viability Assay ^[2]

Cell Line:	3T3-SA and SVEC4-10 cells
Concentration:	0.3, 1 and 3 μM
Incubation Time:	18 hours
Result:	Dose-dependently suppressed TNF-induced death and virus-induced necrosis to improved cell viability.

Appearance

Solid

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL ( 132.45 mM ; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.6491 mL	13.2454 mL	26.4908 mL
5 mM	0.5298 mL	2.6491 mL	5.2982 mL
10 mM	0.2649 mL	1.3245 mL	2.6491 mL

* Please refer to the solubility information to select the appropriate solvent.

In Vivo:

1.

Add each solvent one by one: 10% DMSO >> 90% (20% SBE-β-CD in saline)

Solubility: 4.8 mg/mL (12.72 mM); Suspended solution; Need ultrasonic
2.

Add each solvent one by one: 10% DMSO >> 40% PEG300 >> 5% Tween-80 >> 45% saline

Solubility: ≥ 2.08 mg/mL (5.51 mM); Clear solution

* All of the co-solvents are available by MCE.