[CAS NO. 161804-20-2] Benzamil hydrochloride
PRODUCTS SPECIFICATIONS [161804-20-2]
DESCRIPTION [161804-20-2]
Overview
| MDL | MFCD00069219 |
|---|---|
| Molecular Weight | 356.21 |
| Molecular Formula | C13H15Cl2N7O |
| SMILES | O=C(C1=NC(Cl)=C(N)N=C1N)NC(NCC2=CC=CC=C2)=N.[H]Cl |
Summary
Benzamil hydrochloride (Benzylamiloride hydrochloride), an Amiloride analogue, is a Na + /Ca 2+ exchanger (NCX) inhibitor ( IC 50 ~100 nM). Benzamil hydrochloride also is a non-selective Deg/epithelial sodium channels (ENaC) blocker, and can potentiate myogenic vasoconstriction. Benzamil hydrochloride inhibits TRPP3-mediated Ca 2+ -activated currents, with an IC 50 of 1.1 μM [1] [2] [3] .
In Vitro
Benzamil hydrochloride (Benzylamiloride hydrochloride) inhibits neuronal and heterologously expressed small conductance Ca 2+ -activated K 2+ channels [4] .
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
In Vivo
Benzamil hydrochloride (Benzylamiloride hydrochloride) (0.7 mg/kg/day; s.c.) treated stroke-prone spontaneously hypertensive rats (SHRSP) survived, on average, until 16.1 weeks of age in SHRSP rats [5] .
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Appearance
Solid
Shipping
Room temperature in continental US; may vary elsewhere.
Storage
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
DMSO : ≥ 100 mg/mL ( 280.73 mM )
H 2 O : < 0.1 mg/mL (insoluble)
* "≥" means soluble, but saturation unknown.
Stock Solutions
| Concentration Solvent Mass | 1 mg | 5 mg | 10 mg |
|---|
| 1 mM | 2.8073 mL | 14.0367 mL | 28.0733 mL |
| 5 mM | 0.5615 mL | 2.8073 mL | 5.6147 mL |
| 10 mM | 0.2807 mL | 1.4037 mL | 2.8073 mL |
References
- [1] Fischer KG, et al. Characterization of a Na(+)-Ca(2+) exchanger in podocytes. Nephrol Dial Transplant. 2002 Oct;17(10):1742-50.
- [2] Wang X, et al. Effects of amiloride, benzamil, and alterations in extracellular Na+ on the rat afferent arteriole and its myogenic response. Am J Physiol Renal Physiol. 2008 Jul;295(1):F272-82.
- [3] Dai XQ, et al. Inhibition of TRPP3 channel by amiloride and analogs. Mol Pharmacol. 2007 Dec;72(6):1576-85.
- [4] Castañeda MS, et al. Benzamil inhibits neuronal and heterologously expressed small conductance Ca2+-activated K+channels. Neuropharmacology. 2019 Nov 1;158:107738.
- [5] Teiwes J, et al. Epithelial sodium channel inhibition in cardiovascular disease. A potential role for amiloride. Am J Hypertens. 2007 Jan;20(1):109-17.