[CAS NO. 1644443-92-4]  GW406108X

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PRODUCTS SPECIFICATIONS [1644443-92-4]

Catalog
HY-115570
Brand
MCE
CAS
1644443-92-4

DESCRIPTION [1644443-92-4]

Overview

MDL-
Molecular Weight400.21
Molecular FormulaC20H11Cl2NO4
SMILESO=C1NC2=C(C=C(C(C3=CC=CO3)=O)C=C2)/C1=C/C4=CC(Cl)=C(O)C(Cl)=C4

For research use only. We do not sell to patients.

Summary

GW406108X is a specific Kif15 (Kinesin-12) inhibitor with an IC 50 of 0.82 uM in ATPase assays. GW406108X, a potent autophagy inhibitor, shows ATP competitive inhibition against ULK1 with a pIC 50 of 6.37 (427 nM). GW406108X inhibits ULK1 kinase activity and blocks autophagic flux, without affecting the upstream signaling kinases mTORC1 and AMPK [1] [2] .


In Vitro

GW406108X acts upon ULK1 and autophagy without affecting mTOR or AMPK activity as shown by monitoring ULK1 pS758 (mTOR site) or pS556 (AMPK site) levels. GW406108X inhibits VPS34 and AMPK with pIC 50 of 6.34 (457 nM) and 6.38 (417 nM), respectively. In the presence of 5 µΜ GW406108X, the starvation-induced increase in ATG13 phosphorylation is significantly reduced [1] .
GW406108X inhibits Kif15-N420 ATPase activity by 76% [2] .

MCE has not independently confirmed the accuracy of these methods. They are for reference only.


Appearance

Solid


Shipping

Room temperature in continental US; may vary elsewhere.


Storage

Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL ( 124.93 mM ; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.4987 mL 12.4934 mL 24.9869 mL
5 mM 0.4997 mL 2.4987 mL 4.9974 mL
10 mM 0.2499 mL 1.2493 mL 2.4987 mL
* Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one: 10% DMSO >> 40% PEG300 >> 5% Tween-80 >> 45% saline

    Solubility: 2.08 mg/mL (5.20 mM); Suspended solution; Need ultrasonic

* All of the co-solvents are available by MCE.