[CAS NO. 165682-93-9] O4I2
PRODUCTS SPECIFICATIONS [165682-93-9]
DESCRIPTION [165682-93-9]
Overview
| MDL | MFCD08166530 |
|---|---|
| Molecular Weight | 282.75 |
| Molecular Formula | C12H11ClN2O2S |
| SMILES | O=C(C1=CSC(NC2=CC=C(Cl)C=C2)=N1)OCC |
Summary
In Vitro
O4I2 not only promotes Oct3/4 expression by activating its promoter site, but also stabilize the Oct3/4 protein in HEK293 cells
[1]
.
O4I2 markedly stimulates Oct3/4 even at 5 µM after 72 hrs treatment on the translational
level
[1]
.
O4I2 (20 µM) accumulates Oct3/4 in NCCIT occurred already 2 hrs after incubation, while the detectable Sox2 increase appears much later, implicating compound-mediated activation of Oct3/4 might be different between embryonic kidney HEK293 cells and embryonal carcinoma NCCIT cells
[1]
.
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Immunofluorescence [1]
| Cell Line: | HF (human fibroblasts) cells. |
| Concentration: | 5-20 µM. |
| Incubation Time: | 5 days. |
| Result: | Successfully detected co-expression of Oct3/4 and Sox2, as well as Nanog and Klf4. |
Appearance
Solid
Shipping
Room temperature in continental US; may vary elsewhere.
Storage
| Powder | -20°C | 3 years |
|---|---|---|
| 4°C | 2 years | |
| In solvent | -80°C | 6 months |
| -20°C | 1 month |
Solvent & Solubility
DMSO : ≥ 42 mg/mL ( 148.54 mM )
* "≥" means soluble, but saturation unknown.
Stock Solutions
| Concentration Solvent Mass | 1 mg | 5 mg | 10 mg |
|---|
| 1 mM | 3.5367 mL | 17.6835 mL | 35.3669 mL |
| 5 mM | 0.7073 mL | 3.5367 mL | 7.0734 mL |
| 10 mM | 0.3537 mL | 1.7683 mL | 3.5367 mL |
-
1.
Add each solvent one by one: 10% DMSO >> 90% (20% SBE-β-CD in saline)
Solubility: 2.5 mg/mL (8.84 mM); Suspended solution; Need ultrasonic
Synonyms