[CAS NO. 1818-71-9] Crotonoside
PRODUCTS SPECIFICATIONS [1818-71-9]
DESCRIPTION [1818-71-9]
Overview
| MDL | MFCD04971989 |
|---|---|
| Molecular Weight | 283.24 |
| Molecular Formula | C10H13N5O5 |
| SMILES | OC[C@@H]1[C@H]([C@H]([C@H](N2C=NC(C(N)=N3)=C2NC3=O)O1)O)O |
Summary
IC50 & Target
|
FLT3 |
HDAC3 |
HDAC6 |
In Vitro
Crotonoside (0-200 μM;) selectively inhibits the viability of AML cell line MV4-11, MOLM-13 (with FLT3-ITD mutant) and KG-1 (without FLT3-ITD mutant) in a dose-dependent manner with an IC
50
of 11.6 μM, 12.7 μM and 17.2 μM, respectively
[1]
.
Crotonoside (0-100μM; 7 hours) inhibits the phosphorylation of FLT3 Erk1/2, Akt/mTOR and STAT5 is strongly inhibited by crotonoside at higher concentration of 12.5 μM in a concentration-dependent manner
[1]
.
Crotonoside (0-100 μM; 12 hours) exhibits a dose-dependent increase in the percentage of G0/G1 phase and a dose-dependent decrease in the percentage of G2/M and S phases cells
[1]
.
Crotonoside (0-100 μM; 24 hours) leads to concentration-dependent changes in the number of apoptotic MV4-11 cells, results in a dose-dependent decrease in the level of pro-caspase-3 and a dose-dependent increase in the level of the cleaved caspase-3 fragments
[1]
.
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Cell Viability Assay [1]
| Cell Line: | AML cell line MV4-11, MOLM-13 and KG-1 cells |
| Concentration: | 0-200 μM |
| Incubation Time: | 72 hours |
| Result: | Inhibited AML cells growth than other cell lines tested. |
Western Blot Analysis [1]
| Cell Line: | MV4-11 cells |
| Concentration: | 0 μM, 12.5 μM, 25 μM, 50 μM |
| Incubation Time: | 7 hours |
| Result: | Inhibited AML cells growth than other cell lines tested. |
Cell Cycle Analysis [1]
| Cell Line: | MV4-11 cells |
| Concentration: | 0 μM, 12.5 μM, 25 μM, 50 μM |
| Incubation Time: | 12 hours |
| Result: | Induced cell cycle arrest in G0/G1. |
Apoptosis Analysis [1]
| Cell Line: | MV4-11 cells |
| Concentration: | 0 μM, 12.5 μM, 25 μM, 50 μM |
| Incubation Time: | 24 hours |
| Result: | Induced MV4-11 cell apoptosis. |
In Vivo
Crotonoside(intraperitoneal and intravenous injection; 70 mg/kg, 35 mg/kg; once daily) induces a significant antitumor activity and inhibited xenograft tumor progress as compared to treatment with vehicle
[1]
.
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
| Animal Model: | NOD-SCID mice with MV4-11 cells [1] |
| Dosage: | 70 mg/kg, 35 mg/kg |
| Administration: | Intraperitoneal and intravenous injection; 70 mg/kg, 35 mg/kg; once daily |
| Result: | Produced significant AML tumor inhibition rates of 93.5% at 70 mg/kg. |
Appearance
Solid
Shipping
Room temperature in continental US; may vary elsewhere.
Storage
| Powder | -20°C | 3 years |
|---|---|---|
| 4°C | 2 years | |
| In solvent | -80°C | 6 months |
| -20°C | 1 month |
Solvent & Solubility
DMSO : 25 mg/mL ( 88.26 mM ; Need ultrasonic)
Stock Solutions
| Concentration Solvent Mass | 1 mg | 5 mg | 10 mg |
|---|
| 1 mM | 3.5306 mL | 17.6529 mL | 35.3057 mL |
| 5 mM | 0.7061 mL | 3.5306 mL | 7.0612 mL |
| 10 mM | 0.3531 mL | 1.7653 mL | 3.5306 mL |
Synonyms