[CAS NO. 181885-68-7] LDS-751
PRODUCTS SPECIFICATIONS [181885-68-7]
DESCRIPTION [181885-68-7]
Overview
| MDL | - |
|---|---|
| Molecular Weight | 471.98 |
| Molecular Formula | C25H30ClN3O4 |
| SMILES | CC[N+]1=C2C=CC(N(C)C)=CC2=CC=C1/C=C/C=C/C3=CC=C(N(C)C)C=C3.O=Cl(=O)([O-])=O |
Summary
LDS-751 is a nucleic acid stain principally detecting DNA. LDS-751 has high affinity for DNA and undergoes fluorescence enhancement upon binding, but with maximal emission at 670 nm. LDS-751 and Thiazole orange are used to differentiate erythrocytes, platelets, reticulocytes, and nucleated cells, and both can be excited at 488 nm [1] [2] [3] .
In Vitro
The LDS-751 permits the discrimination of intact cells from residual erythrocyte ghosts, platelets and damaged nucleated cells. The forward and orthogonal fight scattering signals of the intact cells, identified by LDS-751 staining allow a clear separation between the major leukocyte populations since the damaged nucleated cells, erythrocytes, erythrocyte cell ghosts and platelets are removed from the display [1] .
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Appearance
Solid
Shipping
Room temperature in continental US; may vary elsewhere.
Storage
-20°C, sealed storage, away from moisture and light
* The compound is unstable in solutions, freshly prepared is recommended.
Solvent & Solubility
DMSO : 83.33 mg/mL ( 176.55 mM ; Need ultrasonic)
Stock Solutions
| Concentration Solvent Mass | 1 mg | 5 mg | 10 mg |
|---|
| 1 mM | 2.1187 mL | 10.5937 mL | 21.1873 mL |
| 5 mM | 0.4237 mL | 2.1187 mL | 4.2375 mL |
| 10 mM | 0.2119 mL | 1.0594 mL | 2.1187 mL |
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1.
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2.
Add each solvent one by one: 10% DMSO >> 90% (20% SBE-β-CD in saline)
Solubility: ≥ 2.08 mg/mL (4.41 mM); Clear solution
References
- [1] Terstappen LW, et al. A rapid sample preparation technique for flow cytometric analysis of immunofluorescence allowing absolute enumeration of cell subpopulations. J Immunol Methods. 1989 Sep 29;123(1):103-12.
- [2] Terstappen LW, et al. Five-dimensional flow cytometry as a new approach for blood and bone marrow differentials. Cytometry. 1988;9(6):548-556.
- [3] McCarthy DA, et al. A simple flow cytometric procedure for the determination of surface antigens on unfixed leucocytes in whole blood. J Immunol Methods. 1993;163(2):155-160.
Synonyms