[CAS NO. 2070014-99-0]  GNE-617hydrochloride

The activity ofGNE-617 hydrochloride is evaluated on a panel 53 non-small cell lung cancer (NSCLC) cell lines in the presence or absence of 10 μM nicotinic acid. GNE-617 inhibits NAMPT IC50 of 18.9 nM in A549 cell.The majority of cell lines exhibit a steep dose response to GNE-617 when evaluated by decrease in ATP or total nucleic acid, and the cytotoxicity is completely rescued by simultaneous addition of nicotinic acid. The majority of the cell lines tested have IC ₅₀ values below 100 nM, with approximately half with IC ₅₀ values less than 10 nM. Eighteen cell lines are not rescued with nicotinic acid, and these non-rescuable cell lines tended to have lower IC ₅₀ values (P=0.008, Fisher exact test, IC ₅₀ <10 nM vs. ≥10 nM) ^[1] .

MCE has not independently confirmed the accuracy of these methods. They are for reference only.