[CAS NO. 2138882-71-8] KEA1-97
PRODUCTS SPECIFICATIONS [2138882-71-8]
DESCRIPTION [2138882-71-8]
Overview
| MDL | MFCD31697744 |
|---|---|
| Molecular Weight | 335.16 |
| Molecular Formula | C15H9Cl2FN4 |
| SMILES | ClC1=NC(Cl)=NC(NC2=CC=C(C=C2)C3=CC=C(C=C3)F)=N1 |
Summary
IC50 & Target
|
Caspase 3 10 μM (IC 50 ) |
Thioredoxin 10 μM (IC 50 ) |
In Vitro
KEA1-97 (100 μM; 231MFP cells) impaires thioredoxin pulldown of caspase 3
[1]
.
KEA1-97 (10 μM; 48 hours; 231MFP cells) impaires 231MFP serum-free cell proliferation
[1]
.
KEA1-97 (10 μM; 0~12 hours; 231MFP cells) activates caspase 3/7 and induces apoptotic cell death
[1]
.
KEA1-97 (231MFP cells) resistants to survival and proliferation impairments
[1]
.
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Western Blot Analysis [1]
| Cell Line: | 231MFP cells |
| Concentration: | 100 μM |
| Incubation Time: | |
| Result: | Impaired thioredoxin pulldown of caspase 3. |
Cell Proliferation Assay [1]
| Cell Line: | 231MFP cells |
| Concentration: | 10 μM |
| Incubation Time: | 48 h |
| Result: | Impaired 231MFP serum-free cell proliferation. |
Apoptosis Analysis [1]
| Cell Line: | 231MFP cells |
| Concentration: | 10 μM |
| Incubation Time: | 0~12 h |
| Result: | Activated caspase 3/7 and induced apoptotic cell death. |
In Vivo
KEA1-97 (5 mg/kg; i.p.; 50 days) attenuates tumor xenograft growth [1] .
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
| Animal Model: | Immune-deficient SCID mice |
| Dosage: | 5 mg/kg |
| Administration: | I.p.; 50 days |
| Result: | Attenuated tumor xenograft growth. |
Appearance
Solid
Shipping
Room temperature in continental US; may vary elsewhere.
Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
DMSO : 100 mg/mL ( 298.36 mM ; Need ultrasonic)
Stock Solutions
| Concentration Solvent Mass | 1 mg | 5 mg | 10 mg |
|---|
| 1 mM | 2.9836 mL | 14.9182 mL | 29.8365 mL |
| 5 mM | 0.5967 mL | 2.9836 mL | 5.9673 mL |
| 10 mM | 0.2984 mL | 1.4918 mL | 2.9836 mL |
-
1.
Add each solvent one by one: 10% DMSO >> 90% corn oil
Solubility: ≥ 2.5 mg/mL (7.46 mM); Clear solution