- Cart - 0 item(s)
- $0.00
Your shopping cart is empty!
- Chemistry
- Bioactive Molecules
- Anti-infection
- Apoptosis
- Autophagy
- Cell Cycle/DNA Damage
- Cytoskeleton
- Epigenetics
- GPCR/G Protein
- Immunology/Inflammation
- JAK/STAT Signaling
- MAPK/ERK Pathway
- Membrane Transporter/Ion Channel
- Metabolic Enzyme/Protease
- Neuronal Signaling
- NF-κB
- PI3K/Akt/mTOR
- PROTAC
- Protein Tyrosine Kinase/RTK
- Stem Cell/Wnt
- TGF-beta/Smad
- Vitamin D Related/Nuclear Receptor
- Others
- Methylation
- Cytoskeletal Signaling
- Natural Products
- Alkaloids
- Amino acid
- Anthraquinones
- Cerebrosides
- Chalcones
- Coumarins
- Diterpenoids
- Flavonoids
- Iridoids
- Lignans
- Lipids
- Miscellaneous
- Monoterpenoids
- Other terpenes
- Phenols
- Phenylpropanoids
- Quinones
- Saccharides
- Sesquiterpenoids
- Steroids
- Tetraterpenoids
- Thiophenes
- Triterpenoids
- Xanthones
- Polyphenols
- Stibene glucosides
- Oligose
- Anthocyanins
- Miscellaneous
- Chromones
- Terpenoids
- Indoles
- Procyanidins
- Aliphatics
- Botanical Cyclopeptides
- ADCs
- Antibodies
- Multiplex IHC
- Material Science
- Others
[CAS NO. 22144-77-0] Cytochalasin D
| Ships within | Stock | Price | Qty | Total |
Please click "REQUEST A QUOTE" button if there is no stock, or you need other sizes or custom synthesis.
| Catalog: | HY-N6682 |
| Brand: | MCE |
| CAS: | 22144-77-0 |
Overview
| MDL | - |
|---|---|
| Molecular Weight | 507.62 |
| Molecular Formula | C30H37NO6 |
| SMILES | C=C1[C@@H](O)[C@@]2([H])[C@@]3([C@H](OC(C)=O)/C=C/[C@](O)(C)C([C@@H](C)C/C=C/2)=O)[C@@]([C@H](CC4=CC=CC=C4)NC3=O)([H])[C@@H]1C |
18 Publications Citing Use of MCE
- [1]Nat Commun. 2022 Sep 26;13(1):5657.
- [2]Biomaterials. 13 January 2022, 121373.
- [3]Autophagy. 2022 Sep 28;1-20.
- [4]Cell Mol Gastroenterol Hepatol. 2021;11(3):683-696.
- [5]Bioact Mater. 16 August 2022.
- [6]Cell Rep. 2022 Apr 19;39(3):110698.
- [7]Cancer Lett. 2022 Feb 2;215582.
- [8]Mat Sci Eng C-Mater. 2021, 111939.
- [9]Emerg Microbes Infect. 2022 Dec;11(1):1135-1144.
- [10]Stem Cell Res Ther. 2021 Oct 7;12(1):523.
- [11]Front Immunol. 2021 Jun 1;12:684430.
- [12]J Virol. 2022 Nov 23;e0145322.
- [13]Commun Biol. 2022 Mar 24;5(1):262.
- [14]Langmuir. 2020 Sep 29;36(38):11374-11382.
- [15]Cell Mol Biol Lett. 2023 Jan 24;28(1):7.
- [16]Virus Res. 2020 Jan 15;276:197806.
- [17]Lipids Health Dis. 2022 Oct 25;21(1):107.
- [18]Bioengineered. 2022 Apr;13(4):8548-8557.
Summary
Cytochalasin D (Zygosporin A) is a potent actin polymerization inhibitor, could be derived from fungus. Cytochalasin D has cell-permeable activity. Cytochalasin D inhibits the G-actin–cofilin interaction by binding to G-actin. Cytochalasin D also inhibits the binding of cofilin to F-actin and decreases the rate of both actin polymerization and depolymerization in living cells. Cytochalasin D can reduce exosome release, in turn reducing the amount of survivin present in the tumour environment. Cytochalasin D induces phosphorylation and cytoplasmic retention of Yap [1] [2] [3] [4] .
In Vitro
Cytochalasin D (3 and 10 μM; 30 min) causes retraction and arborization of COS-7 cells and conversion of F-actin from long fibers to punctate structures
[1]
.
Cytochalasin D (0.3, 1, 3 and 10 μM; 30 min) concentration-dependently decreases the rate of actin depolymerization in COS-7 cells
[1]
.
Cytochalasin D (1 μM; NIH3T3 cells) disrupts stress fibers, induces phosphorylation of Yap, while cells maintains original area and lost nuclear Yap localization
[4]
.
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Western Blot Analysis
| Cell Line: | COS-7 cells [1] |
| Concentration: | 3 and 10 μM |
| Incubation Time: | 30 min |
| Result: | Caused retraction and arborization of COS-7 cells and conversion of F-actin from long fibers to punctate structures. |
Western Blot Analysis
| Cell Line: | COS-7 cells expressing YFP-actin [1] |
| Concentration: | 0.3, 1, 3 and 10 μM |
| Incubation Time: | 30 min |
| Result: | Concentration-dependently decreased the rate of actin depolymerization in COS-7 cells. |
Clinical Trial
| NCT Number | Sponsor | Condition | Start Date | Phase |
|---|---|---|---|---|
| NCT03677492 | Ibn Sina Hospital |
Infertility
|
September 25, 2018 | Not Applicable |
Appearance
Solid
Source
molds
Shipping
Room temperature in continental US; may vary elsewhere.
Storage
| Powder | -20°C | 3 years |
|---|---|---|
| In solvent | -80°C | 6 months |
| -20°C | 1 month |
Solvent & Solubility
DMSO : 25 mg/mL ( 49.25 mM ; ultrasonic and warming and heat to 60°C)
Stock Solutions
| Concentration Solvent Mass | 1 mg | 5 mg | 10 mg |
|---|
| 1 mM | 1.9700 mL | 9.8499 mL | 19.6998 mL |
| 5 mM | 0.3940 mL | 1.9700 mL | 3.9400 mL |
| 10 mM | 0.1970 mL | 0.9850 mL | 1.9700 mL |
References
- [1] Shoji K, et, al. Cytochalasin D acts as an inhibitor of the actin-cofilin interaction. Biochem Biophys Res Commun. 2012 Jul 20;424(1):52-7.
- [2] Flanagan MD, et, al. Cytochalasins block actin filament elongation by binding to high affinity sites associated with F-actin. J Biol Chem. 1980 Feb 10;255(3):835-8.
- [3] Catalano M, et, al. Inhibiting extracellular vesicles formation and release: a review of EV inhibitors. J Extracell Vesicles. 2019 Dec 19;9(1):1703244.
- [4] Wada K, et, al. Hippo pathway regulation by cell morphology and stress fibers. Development. 2011 Sep;138(18):3907-14.
Synonyms
Arctom is a premier supply platform offering over 600K unique items of Building Blocks, Bioactive Molecules, Natural Products, ADC PEG Linkers, Antibodies, and other Research Chemicals for global pharmaceutical, biotech, university, and industrial customers. We accelerate your research by providing better and faster purchasing experience & services.