[CAS NO. 2253733-10-5] HADAhydrochloride
PRODUCTS SPECIFICATIONS [2253733-10-5]
DESCRIPTION [2253733-10-5]
Overview
| MDL | - |
|---|---|
| Molecular Weight | 328.71 |
| Molecular Formula | C13H13ClN2O6 |
| SMILES | N[C@H](CNC(C1=CC2=CC=C(O)C=C2OC1=O)=O)C(O)=O.[H]Cl |
Summary
HADA hydrochloride (HCC-Amino-D-alanine hydrochloride) is a blue (λ em ~450 nm) fluorescent D-amino acid (FDAA). FDAAs are efficiently incorporated into the peptidoglycans (PGs) of diverse bacterial species at the sites of PG biosynthesis, allowing specific and covalent probing of bacterial growth with minimal perturbation [1] .
In Vitro
FDAA labeling can take as little as 30s for a rapidly growing species such as
Escherichia coli
. Although HADA hydrochloride is dimmer and less photostable than FDL or TDL, it most reproducibly and robustly labels the PG of most bacterial species, typically without the need for extensive optimization
[1]
.
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Appearance
Solid
Shipping
Room temperature in continental US; may vary elsewhere.
Storage
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
DMSO : 125 mg/mL ( 380.27 mM ; Need ultrasonic)
Stock Solutions
| Concentration Solvent Mass | 1 mg | 5 mg | 10 mg |
|---|
| 1 mM | 3.0422 mL | 15.2110 mL | 30.4220 mL |
| 5 mM | 0.6084 mL | 3.0422 mL | 6.0844 mL |
| 10 mM | 0.3042 mL | 1.5211 mL | 3.0422 mL |