[CAS NO. 23062-24-0] Eurycomalactone

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PRODUCTS SPECIFICATIONS [23062-24-0]

Catalog

HY-N4327

Brand

MCE

CAS

23062-24-0

DESCRIPTION [23062-24-0]

Overview

MDL	-
Molecular Weight	348.39
Molecular Formula	C19H24O6
SMILES	C[C@]12[C@@]([C@H]([C@@](O3)([H])[C@@](C)([H])[C@]2([H])C3=O)O)([H])[C@]([C@@](C(C)=CC4=O)([H])CC1=O)([C@@H]4O)C

For research use only. We do not sell to patients.

Summary

Eurycomalactone is an active quassinoid could be isolated from Eurycoma longifolia Jack . Eurycomalactone is a potent NF-κB inhibitor with an IC ₅₀ value of 0.5 μM. Eurycomalactone inhibits protein synthesis and depletes cyclin D1. Eurycomalactone enhances radiosensitivity through arrest cell cycle at G2/M phase and delayed DNA double-strand break repair. Eurycomalactone inhibits the activation of AKT/NF‑κB signaling, induces apoptosis and enhances chemosensitivity to Cisplatin (HY-17394) ^[1] ^[2] ^[3] .

IC50 & Target

IC50: 0.5 μM (NF-κB) ^[1]

In Vitro

Eurycomalactone (24, 48 and 72 h) selectively inhibits the viability of A549 and COR-L23 cells Eurycomalactone inhibits the viability of A549 cells with IC ₅₀ values of 20.17, 3.77, and 1.90 μM for 24, 48 and 72 hours, respectively. Eurycomalactone inhibits the viability of COR-L23 cells with IC ₅₀ values of 25.02, 2.74, and 1.80 μM for 24, 48 and 72 hours, respectively ^[1] .
Eurycomalactone (2.29-156.3 μM; 24 h; A549 and Calu-1 cells) promotes Non-small cell lung cancer (NSCLC) cells apoptosis ^[2] .
Eurycomalactone (0-25.05 μM; 24 h; A549 and COR-L23 cells) induces cell cycle arrest at the radiosensitive G2/M phase and induces apoptosis in irradiated Non-small cell lung cancer (NSCLC) cells. Eurycomalactone downregulated the key G2/M regulatory proteins in irradiated Non-small cell lung cancer (NSCLC) cells ^[1] .
Eurycomalactone (2.5-25 μM; 24 h; A549 cells) suppressed the repair of radiation-Induced DNA double-strand breaks ^[1] .
Eurycomalactone (2.29-156.3 μM; 24 h; A549 and Calu-1 cells) suppresses AKT/NF-κB activation in Non-small cell lung cancer (NSCLC) cells ^[2] .

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Apoptosis Analysis ^[2]

Cell Line:	A549 and Calu-1 cells
Concentration:	2.29, 10.14, 12.02, 20.81, 80.77, and 156.3 μM
Incubation Time:	24 hours
Result:	Increased the apoptotic rates in a dose-dependent manner.

Cell Cycle Analysis ^[1]

Cell Line:	A549 and COR-L23 cells
Concentration:	1.57, 2.57, 20.17 and 25.05 μM
Incubation Time:	24 hours
Result:	Induced cell cycle arrest at G2/M phase in irradiated cells and increased the sub-G1 population. A549 and COR-L23 cells

Western Blot Analysis ^[2]

Cell Line:	A549 and Calu-1 cells
Concentration:	2.29, 10.14, 12.02, 20.81, 80.77, and 156.3 μM
Incubation Time:	24 hours
Result:	Induced the expression levels of active caspase-3 and active PARP (cleaved form), while decreased Bcl-xL and surviving.

Western Blot Analysis ^[1]

Cell Line:	A549 and COR-L23 cells
Concentration:	1.57, 2.57, 20.17 and 25.05 μM
Incubation Time:	24 hours
Result:	Downregulated the expression of both G2/M regulatory proteins in a dose-dependent manner.

Western Blot Analysis ^[2]

Cell Line:	A549 and COR-L23 cells
Concentration:	2.29, 10.14, 12.02, 20.81, 80.77, and 156.3 μM
Incubation Time:	24 hours
Result:	inhibited the expression levels of p ^(S473) -AKT, total AKT, p ^(S536) -NF-κB p65 and total NF-κB p65.

Appearance

Solid

Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL ( 287.03 mM ; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.8703 mL	14.3517 mL	28.7035 mL
5 mM	0.5741 mL	2.8703 mL	5.7407 mL
10 mM	0.2870 mL	1.4352 mL	2.8703 mL

* Please refer to the solubility information to select the appropriate solvent.

References

Synonyms

2,5-Methanonaphth[1,2-d]oxepin-4,6,10(2H)-trione, 1,5,5a,7,7a,11,11a,11b-octahydro-1,11-dihydroxy-5a,8,11a,12-tetramethyl-, (1R,2R,5S,5aR,7aS,11S,11aS,11bR,12R)-

Eurycomalactone

2,5-Methanonaphth[1,2-d]oxepin-4,6,10(2H)-trione, 1,5,5a,7,7a,11,11a,11b-octahydro-1,11-dihydroxy-5a,8,11a,12-tetramethyl-, [1R-(1α,2α,5α,5aα,7aβ,11α,11aα,11bβ,12R*)]-

(1R,2R,5S,5aR,7aS,11S,11aS,11bR,12R)-1,5,5a,7,7a,11,11a,11b-Octahydro-1,11-dihydroxy-5a,8,11a,12-tetramethyl-2,5-methanonaphth[1,2-d]oxepin-4,6,10(2H)-trione