[CAS NO. 2319587-80-7] Vimentin-IN-1
PRODUCTS SPECIFICATIONS [2319587-80-7]
DESCRIPTION [2319587-80-7]
Overview
| MDL | - |
|---|---|
| Molecular Weight | 389.28 |
| Molecular Formula | C19H18Cl2N4O |
| SMILES | COC1=CC(Cl)=CC(N2CCN(C3=C4C=CC=C(Cl)C4=NN=C3)CC2)=C1 |
Summary
Vimentin-IN-1 is a FiVe1 derivative, an orally active and selective anticancer agent. FiVe1 binds type III intermediate filament protein vimentin ( VIM ), to induce hyperphosphorylation of Ser56, resulting selective disruption of mitosis and multinucleation in transformed VIM-expressing mesenchymal cancer cells. Vimentin-IN-1 shows better oral bioavailability and pharmacokinetic profiles than FiVe1 [1] .
IC50 & Target
vimentin (VIM) [1]
In Vitro
Vimentin-IN-1 (compound 4e) (0-10 mM; 72 h) inhibits a marked improvement in potency with an IC
50
value of 44 nM against HT-1080 fibrosarcoma, better than than FiVe1 (IC
50
=1.6 μM, HT-1080)
[1]
.
Vimentin-IN-1 (0.1 μM; 24 h) induces phosphorylation of VIM at Ser56
[1]
.
Vimentin-IN-1 (100 μM; sampled at 0, 5, 15, 30, 45, and 60 min) exhibits poor stability with 0.0% remaining after 60 min of incubation in mouse liver microsome
[1]
.
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Cell Viability Assay [1]
| Cell Line: | HT-1080, RD, and MCF-7 cells |
| Concentration: | 0-10 mM |
| Incubation Time: | 72 hours |
| Result: | Inhibited HT-1080, RD, and MCF-7 cells with IC 50 s of 44 nM, 61 nM, and 49 nM, respectively. |
In Vivo
Vimentin-IN-1 (compound 4e) (10 mg/kg; p.o.; single dose) shows better oral pharmacokinetic properties than Five1
[1]
.
Pharmacokinetic properties of Vimentin-IN-1 in mice
[1]
| Route | Dose (mg/kg) | AUC 0-last (ng·h/mL) | AUC 0-inf (ng·h/mL) | T 1/2 (h) | T max (h) | T last (h) | C max (ng/mL) | |
| 4e | PO | 10 | 371.33 | 534.33 | 4.68 | 0.67 | 8 | 154.67 |
| 4e | IP | 1 | 208.33 | 211.33 | 0.59 | 0.25 | 4 | 197.00 |
| Five1 | PO | 25 | 309.78 | 339.21 | 4.57 | 0.5 | 18 | 110.43 |
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Appearance
Solid
Shipping
Room temperature in continental US; may vary elsewhere.
Storage
| Powder | -20°C | 3 years |
|---|---|---|
| 4°C | 2 years | |
| In solvent | -80°C | 6 months |
| -20°C | 1 month |
Solvent & Solubility
DMSO : 25 mg/mL ( 64.22 mM ; Need ultrasonic)
Stock Solutions
| Concentration Solvent Mass | 1 mg | 5 mg | 10 mg |
|---|
| 1 mM | 2.5688 mL | 12.8442 mL | 25.6885 mL |
| 5 mM | 0.5138 mL | 2.5688 mL | 5.1377 mL |
| 10 mM | 0.2569 mL | 1.2844 mL | 2.5688 mL |
-
1.