[CAS NO. 233605-81-7] AV123
PRODUCTS SPECIFICATIONS [233605-81-7]
DESCRIPTION [233605-81-7]
Overview
| MDL | - |
|---|---|
| Molecular Weight | 234.25 |
| Molecular Formula | C11H14N4O2 |
| SMILES | O=[N+](C1=CC=C2C(NC(NCCCC)=N2)=C1)[O-] |
Summary
AV123 (compound 12) is a non-cytotoxic RIPK1 inhibitor ( IC 50 =12.12 µM). AV123 blocks the TNF-α-induced necroptotic ( EC 50 =1.7 μM) but not the apoptotic cell death. AV123 can be used in the study of necrotic chronic conditions such as ischemia-reperfusion injury of the brain, heart and kidney, inflammatory diseases, neurodegenerative diseases and infectious diseases [1] .
IC50 & Target
IC50: 0.48 µM (CDK9/ CyclinT), 0.80 µM (CLK1 of Mus musculus), 1.80 µM (DYRK1A of Rattus norvegicus), >10 µM (CDK2/CyclinA, CDK5/p25, HASPIN, Pim1, CK1 ε, JAK3, ABL1, RIPK3, AURKB) [1] .
In Vitro
AV123 (0.01-100 µM; 24 h) efficiently blocks necroptosis in a dose-dependent manner in FADD-deficient Jurkat cells
[1]
.
AV123 (0.01-50 µM; 24 h) shows no toxicity to RPE-1 cells
[1]
.
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Cell Viability Assay [1]
| Cell Line: | FADD-deficient Jurkat cells |
| Concentration: | 0.01-100 µM |
| Incubation Time: | 24 h |
| Result: | Significantly blocked the necroptotic cell-death induced by TNF-α with an EC 50 value of 1.7 µM. |
Cell Cytotoxicity Assay [1]
| Cell Line: | RPE-1 cells |
| Concentration: | 0.01-50 µM |
| Incubation Time: | 24 h |
| Result: | Exhibited no toxicity to RPE-1 cells. |
Shipping
Room temperature in continental US; may vary elsewhere.
Storage
Please store the product under the recommended conditions in the Certificate of Analysis.