[CAS NO. 23491-52-3] Hoechst33342

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PRODUCTS SPECIFICATIONS [23491-52-3]

Catalog

HY-15559

Brand

MCE

CAS

23491-52-3

DESCRIPTION [23491-52-3]

Overview

MDL	MFCD00166996
Molecular Weight	452.55
Molecular Formula	C27H28N6O
SMILES	CN1CCN(C2=CC=C3C(N=C(C4=CC=C5C(N=C(C6=CC=C(OCC)C=C6)N5)=C4)N3)=C2)CC1

For research use only. We do not sell to patients.

33 Publications Citing Use of MCE

Summary

Hoechst 33342 is a DNA minor groove binder used fluorochrome for visualizing cellular DNA .

IC50 & Target

Dye reagent ^[1]
DNA Stain ^[1]

In Vitro

Hoechst 33342 binds to adenine-thymine-rich regions of DNA in the minor groove. On binding to DNA, the fluorescence greatly increases. This protocol describes the use of Hoechst 33342 to label nuclear DNA of cells grown in culture. Hoechst 33342 can also be used to stain fixed cells by substituting Hoechst 33342 for DAPI ^[1] .

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Appearance

Solid

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 6.25 mg/mL ( 13.81 mM ; ultrasonic and warming and heat to 60°C)

H ₂ O : 1 mg/mL ( 2.21 mM ; ultrasonic and warming and heat to 80°C)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.2097 mL	11.0485 mL	22.0970 mL
5 mM	0.4419 mL	2.2097 mL	4.4194 mL
10 mM	0.2210 mL	1.1049 mL	2.2097 mL

* Please refer to the solubility information to select the appropriate solvent.

In Vivo:

1.

Add each solvent one by one: PBS

Solubility: 5 mg/mL (11.05 mM); Clear solution; Need ultrasonic and warming and heat to 60°C
2.

Add each solvent one by one: 10% DMSO >> 40% PEG300 >> 5% Tween-80 >> 45% saline

Solubility: ≥ 0.5 mg/mL (1.10 mM); Clear solution
3.

Add each solvent one by one: 10% DMSO >> 90% (20% SBE-β-CD in saline)

Solubility: ≥ 0.5 mg/mL (1.10 mM); Clear solution
4.

Add each solvent one by one: 10% DMSO >> 90% corn oil

Solubility: ≥ 0.5 mg/mL (1.10 mM); Clear solution

* All of the co-solvents are available by MCE.

References

[1] Chazotte B. Labeling nuclear DNA with hoechst 33342. Cold Spring Harb Protoc. 2011 Jan 1;2011(1):pdb.prot5557.

Synonyms

2,6′-Bi-1H-benzimidazole, 2′-(4-ethoxyphenyl)-6-(4-methyl-1-piperazinyl)-

2,5′-Bibenzimidazole, 2′-(p-ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-

2,5′-Bi-1H-benzimidazole, 2′-(4-ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-

2′-(4-Ethoxyphenyl)-6-(4-methyl-1-piperazinyl)-2,6′-bi-1H-benzimidazole

2-[2-(4-Ethoxyphenyl)-6-benzimidazolyl]-6-(1-methyl-4-piperazinyl)benzimidazole

HOE 33342

Hoechst 33342

Bisbenzimide

Ho 342

NSC 334072

BXI 72

Hoechst H 33342

2′-(4-Ethoxyphenyl)-6-(4-methylpiperazin-1-yl)-1H,3′H-2,5′-bibenzimidazole

2-(4-Ethoxyphenyl)-6-[6-(4-methylpiperazin-1-yl)-1H-benzimidazol-2-yl]-1H-benzimidazole