[CAS NO. 2421187-79-1] DiAzKs hydrochloride
PRODUCTS SPECIFICATIONS [2421187-79-1]
DESCRIPTION [2421187-79-1]
Overview
| MDL | - |
|---|---|
| Molecular Weight | 308.76 |
| Molecular Formula | C11H21ClN4O4 |
| SMILES | N[C@@H](CCCCNC(OCCC1(C)N=N1)=O)C(O)=O.[H]Cl |
1 Publications Citing Use of MCE
Summary
DiAzKs (H-L-Photo-lysine) hydrochloride is a diazirine-containing lysine amino acid and is a photo-cross-linker. DiAzKs hydrochloride can site-selective incorporated into proteins and is used to crosslink protein-protein interactions in vitro and in living cells. DiAzKs hydrochloride acts as a UV light-activated photo-crosslinking probe [1] [2] [3] .
In Vitro
Photo-lysine, which is readily incorporated into proteins by native mammalian translation machinery, can be used to capture and identify proteins that recognize lysine post-translational modifications (PTMs), including 'readers' and 'erasers' of histone modifications [2] .
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Appearance
Solid
Shipping
Room temperature in continental US; may vary elsewhere.
Storage
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
DMSO : 250 mg/mL ( 809.69 mM ; Need ultrasonic)
H 2 O : 50 mg/mL ( 161.94 mM ; Need ultrasonic)
Stock Solutions
| Concentration Solvent Mass | 1 mg | 5 mg | 10 mg |
|---|
| 1 mM | 3.2388 mL | 16.1938 mL | 32.3876 mL |
| 5 mM | 0.6478 mL | 3.2388 mL | 6.4775 mL |
| 10 mM | 0.3239 mL | 1.6194 mL | 3.2388 mL |
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1.
Add each solvent one by one: PBS
Solubility: 50 mg/mL (161.94 mM); Clear solution; Need ultrasonic
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2.
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3.
Add each solvent one by one: 10% DMSO >> 90% (20% SBE-β-CD in saline)
Solubility: ≥ 2.08 mg/mL (6.74 mM); Clear solution