[CAS NO. 2519-30-4] BrilliantBlackBN
PRODUCTS SPECIFICATIONS [2519-30-4]
DESCRIPTION [2519-30-4]
Overview
| MDL | MFCD00078980 |
|---|---|
| Molecular Weight | 867.68 |
| Molecular Formula | C28H17N5Na4O14S4 |
| SMILES | O=S(C1=C2C=C(S(=O)(O[Na])=O)C(/N=N/C3=C4C=C(S(=O)(O[Na])=O)C=CC4=C(/N=N/C5=CC=C(S(=O)(O[Na])=O)C=C5)C=C3)=C(O)C2=C(NC(C)=O)C=C1)(O[Na])=O |
Summary
Brilliant black BN (E151) is an azo dye and a food colorant [1] . Brilliant black BN is a promising antiviral agent against EV71 infection via inhibiting the interaction between EV71 and its cellular uncoating factor cyclophilin A. Brilliant black BN has the potential for the investigation of contagious disease [2] .
IC50 & Target
IC50: 2.39 µM- 28.12 µM (various strains of EV71) [2]
In Vitro
Brilliant black BN (E151) inhibits the interaction between EV71 and its cellular uncoating factor cyclophilin A
[1]
.
Brilliant black BN (E151) inhibited the infection EV71 -GFP at a concentration of 300 µM as evidenced from the reduced GFP signals. Brilliant black BN (E151)exhibits the inhibition of EV71 -GFP as a dose-dependent manner in infected RD cells, exhibits an IC
50
value of 10.1 µM
[1]
.
Brilliant black BN (E151) is able to inhibit all tested 28 EV71, CVA16 and CVA6 strains. In rhabdomyosarcoma cells, 50% inhibitory concentration 29 of the dye E151 for various strains of EV71 ranged from 2.39 µM to 28.12 µM ,whereas its 50% cytotoxic concentration is 1870 µM
[1]
.
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
In Vivo
Brilliant black BN (E151)(intraperitoneal injection; 200 mg/kg; 14 days)exhibits mild illness with clinical scores less than 3 and subsequently recovered when compared with PBS-group.All E151 270 treated mice are completely protected throughout the experiment
[1]
.
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
| Animal Model: | 14-days old AG129 mice with EV71 [1] |
| Dosage: | 200 mg/kg |
| Administration: | Intraperitoneal injection |
| Result: | Had a Protective eddect on EV71 challenged AG129 mice by dye E151. |
Appearance
Solid
Shipping
Room temperature in continental US; may vary elsewhere.
Storage
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
DMSO : 125 mg/mL ( 144.06 mM ; Need ultrasonic)
Stock Solutions
| Concentration Solvent Mass | 1 mg | 5 mg | 10 mg |
|---|
| 1 mM | 1.1525 mL | 5.7625 mL | 11.5250 mL |
| 5 mM | 0.2305 mL | 1.1525 mL | 2.3050 mL |
| 10 mM | 0.1152 mL | 0.5762 mL | 1.1525 mL |
References
- [1] Lang W, et al. Biodecolorization of a food azo dye by the deep sea Dermacoccus abyssi MT1.1(T) strain from the Mariana Trench.J Environ Manage. 2014 Jan;132:155-64.
- [2] Meng T, et al. In Vitro and In Vivo Inhibition of the Infectivity of Human Enterovirus 71 by a Sulfonated Food Azo Dye, Brilliant Black BN.J Virol. 2019 Aug 13;93(17).
Synonyms