[CAS NO. 312756-74-4] RS-1
PRODUCTS SPECIFICATIONS [312756-74-4]
DESCRIPTION [312756-74-4]
Overview
| MDL | MFCD00348720 |
|---|---|
| Molecular Weight | 524.23 |
| Molecular Formula | C20H16Br2N2O3S |
| SMILES | O=C(NC1=CC=C(Br)C=C1)C2=CC=C(Br)C(S(=O)(NCC3=CC=CC=C3)=O)=C2 |
1 Publications Citing Use of MCE
Summary
RS-1 is a RAD51 activator, and also increases CRISPR/Cas9 -mediated knock-in efficiencies.
In Vitro
RS-1 is a RAD51 activator, stimulating binding of hRAD51 to DNA with K d ranging from 48 nM to 107 nM in the presence of ATP or ADP and in the absence of a nucleotide cofactor, and such an effect is not via inhibiting its ATPase activity. RS-1 (20 μM) affects the length and helical pitch of hRAD51 protein-DNA complexes. RS-1 (0, 1, 5, 10, 15, 20, and 25 μM) stimulates strand assimilation activity of hRAD51. RS-1 (7.5 μM) promotes resistance of human cells to cross-linking chemotherapy [1] . RS-1 (0, 7.5, 15 μM) increases Cas9-mediated knock-in efficiencies in rabbit embryos [2] .
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Appearance
Solid
Shipping
Room temperature in continental US; may vary elsewhere.
Storage
| Powder | -20°C | 3 years |
|---|---|---|
| 4°C | 2 years | |
| In solvent | -80°C | 6 months |
| -20°C | 1 month |
Solvent & Solubility
DMSO : ≥ 100 mg/mL ( 190.76 mM )
* "≥" means soluble, but saturation unknown.
Stock Solutions
| Concentration Solvent Mass | 1 mg | 5 mg | 10 mg |
|---|
| 1 mM | 1.9076 mL | 9.5378 mL | 19.0756 mL |
| 5 mM | 0.3815 mL | 1.9076 mL | 3.8151 mL |
| 10 mM | 0.1908 mL | 0.9538 mL | 1.9076 mL |
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Add each solvent one by one: 10% DMSO >> 90% corn oil
Solubility: ≥ 3 mg/mL (5.72 mM); Clear solution
References