[CAS NO. 32502-62-8] 3-Methyltoxoflavin
PRODUCTS SPECIFICATIONS [32502-62-8]
DESCRIPTION [32502-62-8]
Overview
| MDL | MFCD00449892 |
|---|---|
| Molecular Weight | 207.19 |
| Molecular Formula | C8H9N5O2 |
| SMILES | CC1=NN(C)C(C2=N1)=NC(N(C)C2=O)=O |
Summary
3-Methyltoxoflavin is a potent Protein disulfide isomerase (PDI) inhibitor, with an IC 50 of 170 nM.
IC50 & Target
IC50: 170 nM (PDI) [1] .
In Vitro
3-Methyltoxoflavin is a potent Protein disulfide isomerase (PDI) inhibitor, with an IC 50 of 170 nM. 3-Methyltoxoflavin is toxic in a panel of human glioblastoma cell lines. From the screen, 3-Methyltoxoflavin emerges as the most cytotoxic inhibitor of PDI. Bromouridine labeling and sequencing (Bru-seq) of nascent RNA reveals that 3-Methyltoxoflavin induces Nrf2 antioxidant response, ER stress response, and autophagy. Specifically, 3-Methyltoxoflavin upregulates heme oxygenase 1 and SLC7A11 transcription and protein expression and represses PDI target genes such as TXNIP and EGR1. Interestingly, 3-Methyltoxoflavin-induced cell death does not proceed via apoptosis or necrosis, but by a mixture of autophagy and ferroptosis [1] .
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Appearance
Solid
Shipping
Room temperature in continental US; may vary elsewhere.
Storage
| Powder | -20°C | 3 years |
|---|---|---|
| 4°C | 2 years | |
| In solvent | -80°C | 6 months |
| -20°C | 1 month |
Solvent & Solubility
DMSO : 50 mg/mL ( 241.32 mM ; ultrasonic and warming and heat to 60°C)
Stock Solutions
| Concentration Solvent Mass | 1 mg | 5 mg | 10 mg |
|---|
| 1 mM | 4.8265 mL | 24.1324 mL | 48.2649 mL |
| 5 mM | 0.9653 mL | 4.8265 mL | 9.6530 mL |
| 10 mM | 0.4826 mL | 2.4132 mL | 4.8265 mL |