[CAS NO. 329689-23-8] Monastrol
PRODUCTS SPECIFICATIONS [329689-23-8]
DESCRIPTION [329689-23-8]
Overview
| MDL | MFCD00813077 |
|---|---|
| Molecular Weight | 292.35 |
| Molecular Formula | C14H16N2O3S |
| SMILES | O=C(C1=C(C)NC(NC1C2=CC=CC(O)=C2)=S)OCC |
2 Publications Citing Use of MCE
Summary
Monastrol is a potent and cell-permeable inhibitor of the mitotic kinesin Eg5 with an IC 50 value of 14 μM.
IC50 & Target
|
Eg5 14 μM (IC 50 ) |
In Vitro
Monastrol is a small, cell-permeable molecule that arrests cells in mitosis by specifically inhibiting Eg5, a member of the Kinesin-5 family. Monastrol treatment of dividing cells results in spindle collapse and cell cycle arrest with a monoastral spindle, which is similar to the phenotype observed when Eg5 is inhibited by anti-Eg5 antibodies [1] . Monastrol is an allosteric inhibitor of the mitotic kinesin Eg5 that exhibits an antiproliferative effect against several cell lines. Monastrol treatment can decrease cell viability in MCF-7 tumor cells. Real-time cell growth kinetic analysis showed a decrease in the proliferation of MCF-7 cells exposed to monastrol [2] .
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Appearance
Solid
Shipping
Room temperature in continental US; may vary elsewhere.
Storage
| Powder | -20°C | 3 years |
|---|---|---|
| 4°C | 2 years | |
| In solvent | -80°C | 6 months |
| -20°C | 1 month |
Solvent & Solubility
DMSO : ≥ 33 mg/mL ( 112.88 mM )
* "≥" means soluble, but saturation unknown.
Stock Solutions
| Concentration Solvent Mass | 1 mg | 5 mg | 10 mg |
|---|
| 1 mM | 3.4206 mL | 17.1028 mL | 34.2056 mL |
| 5 mM | 0.6841 mL | 3.4206 mL | 6.8411 mL |
| 10 mM | 0.3421 mL | 1.7103 mL | 3.4206 mL |
References
- [1] Cochran JC, et al. Monastrol inhibition of the mitotic kinesin Eg5. J BiolChem. 2005 Apr 1;280(13):12658-67.
- [2] Mayer TU, et al. Small molecule inhibitor of mitotic spindle bipolarity identified in a phenotype-based screen. Science. 1999 Oct 29;286(5441):971-4.
- [3] Marques LA, et al. Antiproliferative activity of monastrol in human adenocarcinoma (MCF-7) and non-tumor (HB4a) breast cells. Naunyn Schmiedebergs Arch Pharmacol. 2016 Dec;389(12):1279-1288.
Synonyms