[CAS NO. 331244-89-4] BAM7
PRODUCTS SPECIFICATIONS [331244-89-4]
DESCRIPTION [331244-89-4]
Overview
| MDL | MFCD01246556 |
|---|---|
| Molecular Weight | 405.47 |
| Molecular Formula | C21H19N5O2S |
| SMILES | CCOC1=CC=CC=C1N/N=C2C(C)=NN(C3=NC(C4=CC=CC=C4)=CS3)C\2=O |
2 Publications Citing Use of MCE
Summary
BAM7 is a direct and selective activator of proapoptotic BAX with an IC 50 of 3.3 μM.
IC50 & Target
|
Bax 3.3 μM (IC 50 ) |
In Vitro
BAM7 is selective for the BH3-binding site on BAX. BAM7 activates BAX and BAX-dependent cell death. Whereas treatment with BAX or BAM7 alone has no effect on the liposomes, the combination of BAM7 and BAX yields dose-responsive liposomal release of entrapped fluorophore. BAM7 dose- and time-responsively impairs the viability of Bak -/- MEFs that exclusively express BAX but has no effect on Bak -/- MEFs that contain BAK but lack BAX. In contrast, standard proapoptotic stimuli such as serum withdrawal, Staurosporine and Etoposide induces an equivalent apoptotic response in Bax -/- and Bak -/- MEFs. As further evidence of BAM7 specificity of action, (i) BAM7 does not affect the viability of Bax -/- Bak -/- MEFs; (ii) ANA-BAM16, which does not bind or activate BAX, has no effect on Bak -/- MEFs; and (iii) BAM7 selectively induces cell death of Bax -/- Bak -/- MEFs reconstituted with wild-type BAX but not BAXK21E , which bears the mutation that abrogates BAM7 binding [1] .
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Appearance
Solid
Shipping
Room temperature in continental US; may vary elsewhere.
Storage
| Powder | -20°C | 3 years |
|---|---|---|
| 4°C | 2 years | |
| In solvent | -80°C | 6 months |
| -20°C | 1 month |
Solvent & Solubility
DMSO : 5 mg/mL ( 12.33 mM ; Need ultrasonic)
Stock Solutions
| Concentration Solvent Mass | 1 mg | 5 mg | 10 mg |
|---|
| 1 mM | 2.4663 mL | 12.3314 mL | 24.6627 mL |
| 5 mM | 0.4933 mL | 2.4663 mL | 4.9325 mL |
| 10 mM | 0.2466 mL | 1.2331 mL | 2.4663 mL |
-
1.
Add each solvent one by one: 10% DMSO >> 90% corn oil
Solubility: ≥ 0.5 mg/mL (1.23 mM); Clear solution