[CAS NO. 340316-62-3] SMIFH2
PRODUCTS SPECIFICATIONS [340316-62-3]
DESCRIPTION [340316-62-3]
Overview
| MDL | - |
|---|---|
| Molecular Weight | 377.21 |
| Molecular Formula | C15H9BrN2O3S |
| SMILES | O=C(/C(C(N1C2=CC=CC(Br)=C2)=O)=C\C3=CC=CO3)NC1=S |
Summary
SMIFH2 is a formin specific inhibitor. SMIFH2 inhibits actin polymerization by Formins and affects the actin cytoskeleton [1] .
IC50 & Target
Formin [1]
In Vitro
SMIFH2 (25 uM; 1-16 hours) induces dynamic cytoskeletal remodelling in U2OS cells
[1]
.
SMIFH2 (25 μM) reduces p300, mDia2 and p53 levels in a proteasome-independent manner
[1]
.
SMIFH2 reduces expression and activity of p53 through a post-transcriptional, proteasome-independent mechanism that influences remodelling of the cytoskeleton
[1]
.
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Western Blot Analysis [1]
| Cell Line: | 293T, A375, U2OS, and MDA-MB-231 cells |
| Concentration: | 25 μM |
| Incubation Time: | 5 hours for 293T, U2OS, MDA-MB-231 cells; 2.5 hours for A375 cells |
| Result: | Downregulated mDia2, p53 and p300 protein levels. |
Appearance
Solid
Shipping
Room temperature in continental US; may vary elsewhere.
Storage
| Powder | -20°C | 3 years |
|---|---|---|
| 4°C | 2 years | |
| In solvent | -80°C | 6 months |
| -20°C | 1 month |
Solvent & Solubility
DMSO : 125 mg/mL ( 331.38 mM ; Need ultrasonic)
Stock Solutions
| Concentration Solvent Mass | 1 mg | 5 mg | 10 mg |
|---|
| 1 mM | 2.6510 mL | 13.2552 mL | 26.5104 mL |
| 5 mM | 0.5302 mL | 2.6510 mL | 5.3021 mL |
| 10 mM | 0.2651 mL | 1.3255 mL | 2.6510 mL |
-
1.
Add each solvent one by one: 10% DMSO >> 90% (20% SBE-β-CD in saline)
Solubility: 2.08 mg/mL (5.51 mM); Suspended solution; Need ultrasonic