[CAS NO. 375835-43-1] Necrostatin-34
PRODUCTS SPECIFICATIONS [375835-43-1]
DESCRIPTION [375835-43-1]
Overview
| MDL | - |
|---|---|
| Molecular Weight | 384.48 |
| Molecular Formula | C18H16N4O2S2 |
| SMILES | O=C(NC1=NC=CS1)CSC2=C(C#N)C(C3=CC=C(C)C=C3)CC(N2)=O |
Summary
In Vitro
Necrostatin-34 (Nec-34) exhibits IC
50
values of 667 nM and 134 nM for TNFα in FADD def-Jurkat cells and L939 cells, respectively
[1]
.
Necrostatin-34 (Nec-34, 10 μM) inhibits the dimerization-induced RIPK1 activation as examined by phosphorylation of Ser166 (p-S166) of RIPK1, a biomarker for RIPK1 activation
[1]
.
Necrostatin-34 (Nec-34) may block TNFα-induced complex II formation by inhibiting the activation of RIPK1 kinase
[1]
.
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Western Blot Analysis [1]
| Cell Line: | RIPK1 knockout L929 cells transfected with an expressing vector encoding an inducible and dimerizable RIPK1 fused with FKBP at the C-terminus. |
| Concentration: | 10 μM. |
| Incubation Time: | 30 min (and then 100 ng/mL TNFα was added for indicated periods of time). |
| Result: | Downregulated p-S166 levels. |
Appearance
Solid
Shipping
Room temperature in continental US; may vary elsewhere.
Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
DMSO : 125 mg/mL ( 325.11 mM ; Need ultrasonic)
Stock Solutions
| Concentration Solvent Mass | 1 mg | 5 mg | 10 mg |
|---|
| 1 mM | 2.6009 mL | 13.0046 mL | 26.0092 mL |
| 5 mM | 0.5202 mL | 2.6009 mL | 5.2018 mL |
| 10 mM | 0.2601 mL | 1.3005 mL | 2.6009 mL |
-
1.
-
2.
Add each solvent one by one: 10% DMSO >> 90% corn oil
Solubility: ≥ 2.08 mg/mL (5.41 mM); Clear solution