[CAS NO. 394694-98-5] MitoBloCK-10
PRODUCTS SPECIFICATIONS [394694-98-5]
DESCRIPTION [394694-98-5]
Overview
| MDL | - |
|---|---|
| Molecular Weight | 293.27 |
| Molecular Formula | C12H8FN3O3S |
| SMILES | O=C(N/N=C/C1=CC=C([N+]([O-])=O)S1)C2=CC=CC(F)=C2 |
Summary
In Vitro
MB-10 (100 μM) is a potential attenuator of protein import into mitochondria
[1]
.
MB-10 inhibits the import of substrates that use the TIM23 import pathway
[1]
.
MB-10 (0-100 μM) inhibits protein import into mammalian mitochondria
[1]
.
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Cell Viability Assay [1]
| Cell Line: | A plasmid expressing Su9-Ura3-myc was integrated at the LEU2 locus in WT and tim23-2 strains. |
| Concentration: | 100 μM. |
| Incubation Time: | 30 min. |
| Result: | WT strain expressing Su9-Ura3 failed to grow, whereas the tim23-2 mutant expressing Su9-Ura3 grew much faster. The strains grew at a similar rate when the medium was supplemented with uracil. |
Cell Viability Assay [1]
| Cell Line: | HeLa cells. |
| Concentration: | 0-100 μM. |
| Incubation Time: | 24 h. |
| Result: | Inhibited HeLa cells vibility, with an IC 50 of 17.2 μM. |
Appearance
Solid
Shipping
Room temperature in continental US; may vary elsewhere.
Storage
| Powder | -20°C | 3 years |
|---|---|---|
| 4°C | 2 years | |
| In solvent | -80°C | 6 months |
| -20°C | 1 month |
Solvent & Solubility
DMSO : 125 mg/mL ( 426.23 mM ; Need ultrasonic)
Stock Solutions
| Concentration Solvent Mass | 1 mg | 5 mg | 10 mg |
|---|
| 1 mM | 3.4098 mL | 17.0491 mL | 34.0983 mL |
| 5 mM | 0.6820 mL | 3.4098 mL | 6.8197 mL |
| 10 mM | 0.3410 mL | 1.7049 mL | 3.4098 mL |
-
1.
Add each solvent one by one: 10% DMSO >> 90% (20% SBE-β-CD in saline)
Solubility: 2.08 mg/mL (7.09 mM); Suspended solution; Need ultrasonic