[CAS NO. 415713-60-9] RI-1

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PRODUCTS SPECIFICATIONS [415713-60-9]

Catalog

HY-15317

Brand

MCE

CAS

415713-60-9

DESCRIPTION [415713-60-9]

Overview

MDL	MFCD00814248
Molecular Weight	361.61
Molecular Formula	C14H11Cl3N2O3
SMILES	O=C(C(N1CCOCC1)=C2Cl)N(C3=CC=C(Cl)C(Cl)=C3)C2=O

For research use only. We do not sell to patients.

4 Publications Citing Use of MCE

Summary

RI-1 is a RAD51 inhibitor, with IC ₅₀ s ranging from 5 to 30 μM. RI-1 binds covalently to the surface of RAD51 protein at cysteine 319. RI-1 inactivates RAD51 by directly binding to a protein surface that serves as an interface between protein subunits in RAD51 filaments. RI-1 can disrupt homologous recombination in human cells ^[1] .

IC50 & Target

IC50: 5-30 μM (RAD51) ^[1]

In Vitro

RI-1 (1-50 µM; 24 h) specifically inhibits homologous recombination (HR) in U2OS cells and stimulates single-strand annealing (SSA) in HEK293 cells ^[1] .
RI-1 (5-20 µM; 30 min) inhibits HsRAD51 in a concentration-dependent manner ^[1] .
RI-1 (20 µM; 8 h) disrupts the formation of RAD51 foci after DNA damage in immortalized human fibroblasts ^[1] .
RI-1 (15-25 µM; 24 h) sensitizes human cancer cells (HeLa, MCF-7 and U2OS) to cross-linking chemotherapy ^[1] .

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

RI-1 (50 mg/kg; i.p. every 3 d for 30 d) significantly reduces triple negative breast cancer (TNBC) tumor growth in mice ^[2] .

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Female BALB/c nude mice (6 weeks) bearing TNBC tumor ^[2]
Dosage:	50 mg/kg
Administration:	I.p. every 3 days for 30 days
Result:	Resulted in significant inhibition of tumor growth. Did not cause body weight loss significantly.

Appearance

Solid

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 33.33 mg/mL ( 92.17 mM ; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.7654 mL	13.8271 mL	27.6541 mL
5 mM	0.5531 mL	2.7654 mL	5.5308 mL
10 mM	0.2765 mL	1.3827 mL	2.7654 mL

* Please refer to the solubility information to select the appropriate solvent.

In Vivo:

1.

Add each solvent one by one: 10% DMSO >> 90% corn oil

Solubility: ≥ 2.5 mg/mL (6.91 mM); Clear solution

* All of the co-solvents are available by MCE.

References

Synonyms

1H-Pyrrole-2,5-dione, 3-chloro-1-(3,4-dichlorophenyl)-4-(4-morpholinyl)-

3-Chloro-1-(3,4-dichlorophenyl)-4-(4-morpholinyl)-1H-pyrrole-2,5-dione

RI 1

3-Chloro-1-(3,4-dichlorophenyl)-4-morpholin-4-ylpyrrole-2,5-dione

3-Chloro-1-(3,4-dichlorophenyl)-4-morpholino-1H-pyrrole-2,5-dione