[CAS NO. 41906-86-9] Nitrocefin

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PRODUCTS SPECIFICATIONS [41906-86-9]

Catalog

HY-108913

Brand

MCE

CAS

41906-86-9

DESCRIPTION [41906-86-9]

Overview

MDL	-
Molecular Weight	516.50
Molecular Formula	C21H16N4O8S2
SMILES	O=C(C(N12)=C(/C=C/C3=CC=C([N+]([O-])=O)C=C3[N+]([O-])=O)CS[C@]2([H])[C@H](NC(CC4=CC=CS4)=O)C1=O)O

For research use only. We do not sell to patients.

Summary

Nitrocefin is a chromogenic β-lactamase substrate that undergoes distinctive color change from yellow to red as the amide bond in the β-lactam ring is hydrolyzed by β-lactamase. Nitrocefin is used in competitive inhibition studies in developmental work on β-lactamase-resistant antibiotics ^[1] .

Appearance

Solid

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL ( 193.61 mM ; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.9361 mL	9.6805 mL	19.3611 mL
5 mM	0.3872 mL	1.9361 mL	3.8722 mL
10 mM	0.1936 mL	0.9681 mL	1.9361 mL

* Please refer to the solubility information to select the appropriate solvent.

References

[1] Shannon K, Phillips I. beta-Lactamase detection by three simple methods: Intralactam, nitrocefin and acidimetric. J Antimicrob Chemother. 1980 Sep;6(5):617-21.

Synonyms

5-Thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid, 3-[(1E)-2-(2,4-dinitrophenyl)ethenyl]-8-oxo-7-[(2-thienylacetyl)amino]-, (6R,7R)-

5-Thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid, 3-[2-(2,4-dinitrophenyl)ethenyl]-8-oxo-7-[(2-thienylacetyl)amino]-, [6R-[3(E),6α,7β]]-

(6R,7R)-3-[(1E)-2-(2,4-Dinitrophenyl)ethenyl]-8-oxo-7-[(2-thienylacetyl)amino]-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid

Nitrocefin