[CAS NO. 442632-72-6] CK-636
PRODUCTS SPECIFICATIONS [442632-72-6]
DESCRIPTION [442632-72-6]
Overview
| MDL | MFCD03036245 |
|---|---|
| Molecular Weight | 284.38 |
| Molecular Formula | C16H16N2OS |
| SMILES | O=C(C1=CC=CS1)NCCC2=C(C)NC3=C2C=CC=C3 |
1 Publications Citing Use of MCE
Summary
CK-636 is a cell permeable inhibitor of Arp2/3 complex , that could inhibit actin polymerization, with IC 50 values of 4 μM, 24 μM and 32 μM for human, fission yeast and bovine, respectively. CK636 blocks cell migration.
IC50 & Target
IC50: 4/24/32 µM (Human/fission yeast/bovine Arp2/3) [1] .
In Vitro
CK-636 binds between Arp2 and Arp3, where it appears to block movement of Arp2 and Arp3 into their active conformation. CK-636 inserts into the hydrophobic core of Arp3 and alters its conformation. CK-636 prevents actin polymerization and the formation of actin filament comet tails by Listeria in infected SKOV3 cells (IC 50 =22 µM) [1] . Additionally, CK-636-treated T cells exhibits elongated morphology with sharp pseudopodia at the leading edges, while the breadth of the CK-636-treated T cells is about 30% less than that of DMSO-treated T cells [2] .
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Appearance
Solid
Shipping
Room temperature in continental US; may vary elsewhere.
Storage
| Powder | -20°C | 3 years |
|---|---|---|
| 4°C | 2 years | |
| In solvent | -80°C | 6 months |
| -20°C | 1 month |
Solvent & Solubility
DMSO : ≥ 49 mg/mL ( 172.30 mM )
* "≥" means soluble, but saturation unknown.
Stock Solutions
| Concentration Solvent Mass | 1 mg | 5 mg | 10 mg |
|---|
| 1 mM | 3.5164 mL | 17.5821 mL | 35.1642 mL |
| 5 mM | 0.7033 mL | 3.5164 mL | 7.0328 mL |
| 10 mM | 0.3516 mL | 1.7582 mL | 3.5164 mL |
References
- [1] Nolen BJ, et al. Characterization of two classes of small molecule inhibitors of Arp2/3 complex. Nature. 2009 Aug 20;460(7258):1031-4.
- [2] Kwon KW, et al. Migration of T cells on surfaces containing complex nanotopography. PLoS One. 2013 Sep 12;8(9):e73960.
- [3] Liang Ma, et al. Discovery of the migrasome, an organelle mediating release of cytoplasmic contents during cell migration. Cell Res. 2015 Jan;25(1):24-38.