[CAS NO. 442633-00-3] CK-666
PRODUCTS SPECIFICATIONS [442633-00-3]
DESCRIPTION [442633-00-3]
Overview
| MDL | - |
|---|---|
| Molecular Weight | 296.34 |
| Molecular Formula | C18H17FN2O |
| SMILES | O=C(NCCC1=C(C)NC2=C1C=CC=C2)C3=CC=CC=C3F |
4 Publications Citing Use of MCE
Summary
CK-666 is a cell-permeable actin-related protein Arp2/3 complex inhibitor ( IC 50 =12 μM). CK-666 binds to Arp2/3 complex , stabilizes the inactive state of the complex, blocking movement of the Arp2 and Arp3 subunits into the activated filament-like (short pitch) conformation [1] [2] .
IC50 & Target
|
HIV-1 |
In Vitro
CK-666 (100 μM) significantly reduces the number of filopodia on the cell surface of treated trabecular meshwork (TM) cells as well as reduces the length of those filopodia that are present [3] .
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Appearance
Solid
Shipping
Room temperature in continental US; may vary elsewhere.
Storage
| Powder | -20°C | 3 years |
|---|---|---|
| 4°C | 2 years | |
| In solvent | -80°C | 6 months |
| -20°C | 1 month |
Solvent & Solubility
DMSO : 125 mg/mL ( 421.81 mM ; Need ultrasonic)
Stock Solutions
| Concentration Solvent Mass | 1 mg | 5 mg | 10 mg |
|---|
| 1 mM | 3.3745 mL | 16.8725 mL | 33.7450 mL |
| 5 mM | 0.6749 mL | 3.3745 mL | 6.7490 mL |
| 10 mM | 0.3375 mL | 1.6873 mL | 3.3745 mL |
References
- [1] Hetrick B, et al. Small molecules CK-666 and CK-869 inhibit actin-related protein 2/3 complex by blocking an activating conformational change. Chem Biol. 2013 May 23;20(5):701-12.
- [2] Brieuc P Perot, et al. Dendritic Cell Maturation Regulates TSPAN7 Function in HIV-1 Transfer to CD4 T Lymphocytes. Front Cell Infect Microbiol. 2020 Feb 28;10:70.