[CAS NO. 442644-28-2] JW67
PRODUCTS SPECIFICATIONS [442644-28-2]
DESCRIPTION [442644-28-2]
Overview
| MDL | - |
|---|---|
| Molecular Weight | 394.38 |
| Molecular Formula | C21H18N2O6 |
| SMILES | O=C1C2(OCC3(COC4(C5=CC=CC=C5NC4=O)OC3)CO2)C6=CC=CC=C6N1 |
Summary
JW67 inhibits the canonical Wnt signaling with an IC 50 of 1.17μM [1] . JW67 affects the multiprotein complex consisting of β-catenin/GSK-3β/AXIN/APC/CK1 that rapidly reduces active β-catenin with a subsequent downregulation of Wnt target genes. JW67 also inhibits colorectal cancer cell growth [1] .
IC50 & Target
IC50: 1.17μM (Wnt signaling) [1]
In Vitro
JW67 (1 μM; 24 hours) increases concentration of AXIN2 and decreases the active form of β-catenin
[1]
.
JW67 affects the expression of Wnt target genes and reduces cell growth of colon cancer cell lines
[1]
.
JW67 shows a concentrationdependent reduction in proliferation with a GI50 value of 7.8 μM
[1]
.
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Western Blot Analysis [1]
| Cell Line: | SW480 cells |
| Concentration: | 1 μM |
| Incubation Time: | 24 hours |
| Result: | Increased the AXIN2 expression. |
RT-PCR [1]
| Cell Line: | SW480 cells |
| Concentration: | 10 or 25μM |
| Incubation Time: | 72 hours |
| Result: | Reduced growth of SW480 CRC cells in vitro by inhibiting cell-cycle progression at the G1/S. |
Appearance
Solid
Shipping
Room temperature in continental US; may vary elsewhere.
Storage
| Powder | -20°C | 3 years |
|---|---|---|
| 4°C | 2 years | |
| In solvent | -80°C | 6 months |
| -20°C | 1 month |
Solvent & Solubility
DMSO : 50 mg/mL ( 126.78 mM ; Need ultrasonic)
Stock Solutions
| Concentration Solvent Mass | 1 mg | 5 mg | 10 mg |
|---|
| 1 mM | 2.5356 mL | 12.6781 mL | 25.3563 mL |
| 5 mM | 0.5071 mL | 2.5356 mL | 5.0713 mL |
| 10 mM | 0.2536 mL | 1.2678 mL | 2.5356 mL |
-
1.
-
2.
Add each solvent one by one: 10% DMSO >> 90% (20% SBE-β-CD in saline)
Solubility: ≥ 2.5 mg/mL (6.34 mM); Clear solution