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[CAS NO. 465-21-4] Bufalin
| Ships within | Stock | Price | Qty | Total |
Please click "REQUEST A QUOTE" button if there is no stock, or you need other sizes or custom synthesis.
| Catalog: | HY-N0877 |
| Brand: | MCE |
| CAS: | 465-21-4 |
Overview
| MDL | MFCD00056525 |
|---|---|
| Molecular Weight | 386.52 |
| Molecular Formula | C24H34O4 |
| SMILES | O[C@]([C@@](CC[C@@]1([H])[C@@]2(CC[C@H](O)C1)C)([H])[C@]2([H])CC3)(CC[C@@H]4C(C=C5)=COC5=O)[C@]34C |
5 Publications Citing Use of MCE
Summary
IC50 & Target
Kd: 42.5 nM (Na,K-ATPase α1), 45 nM (Na,K-ATPase α2), 40 nM (Na,K-ATPase α3) [1]
In Vitro
Bufalin (0, 1, 2, 4 μM for 48 hours) decreases cell viability in NCI-H460 cells
[2]
.
Bufalin (2 μM) increases caspae-3, Endo G and GADD153 mRNA expression, but decreases the GRP78 mRNA expression
[2]
.
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Cell Viability Assay [2]
| Cell Line: | NCI-H460 cells |
| Concentration: | 0, 1, 2, 4 μM |
| Incubation Time: | 48 hours |
| Result: | Decreased viability of NCI-H460 cells in a dose-dependent manner. |
In Vivo
Bufalin (0.1, 0.2, or 0.4 mg/kg, i.p., daily for 14 days) shows significant anti-tumor activity in mice bearing NCI-H460 cells [2] .
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
| Animal Model: | Forty male athymic BALB/c nu/nu mice (6-8 weeks old) [2] |
| Dosage: | 0.1, 0.2, or 0.4 mg/kg |
| Administration: | I.P. every day until 14 days |
| Result: | Dose-dependently suppressed tumor growth. |
Appearance
Solid
Source
toad
Shipping
Room temperature in continental US; may vary elsewhere.
Storage
| Powder | -20°C | 3 years |
|---|---|---|
| 4°C | 2 years | |
| In solvent | -80°C | 6 months |
| -20°C | 1 month |
Solvent & Solubility
DMSO : 100 mg/mL ( 258.72 mM ; Need ultrasonic)
Stock Solutions
| Concentration Solvent Mass | 1 mg | 5 mg | 10 mg |
|---|
| 1 mM | 2.5872 mL | 12.9359 mL | 25.8719 mL |
| 5 mM | 0.5174 mL | 2.5872 mL | 5.1744 mL |
| 10 mM | 0.2587 mL | 1.2936 mL | 2.5872 mL |
References
- [1] Katz A, et al. Selectivity of digitalis glycosides for isoforms of human Na,K-ATPase. J Biol Chem. 2010 Jun 18;285(25):19582-92.
- [2] Wu SH, et al. Bufalin induces apoptosis in vitro and has Antitumor activity against human lung cancer xenografts in vivo. Environ Toxicol. 2017 Apr;32(4):1305-1317.
Synonyms
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