[CAS NO. 6019-39-2] OAC2
PRODUCTS SPECIFICATIONS [6019-39-2]
DESCRIPTION [6019-39-2]
Overview
| MDL | MFCD03055811 |
|---|---|
| Molecular Weight | 236.27 |
| Molecular Formula | C15H12N2O |
| SMILES | O=C(NC1=CC2=C(NC=C2)C=C1)C3=CC=CC=C3 |
Summary
OAC2 is an Oct4-activating compound which activates expression through the Oct4 gene promoter.
In Vitro
Octamer-binding transcription factor 4 (Oct4) is a master regulator of the induction and maintenance of cellular pluripotency, and has crucial roles in early stages of differentiation. It is the only factor that cannot be substituted by other members of the same protein family to induce pluripotency [1] . Oct4 has been shown to be an essential regulator of embryonic stem cell (ESC) pluripotency and key to the reprogramming process. OAC2 is a structural analog of OAC1. OAC2 activates both Oct4 and Nanog reporters to a similar extent as OAC1. OAC1 and its two structural analogs OAC2 and OAC3 enhances reprogramming efficiency fourfold, up to as high as 2.75%, and accelerates the appearance of iPSC colonies 3 to 4 d when used in combination with the four reprogramming factors, Oct4, Sox2, Klf4, and c-Myc [2] .
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Appearance
Solid
Shipping
Room temperature in continental US; may vary elsewhere.
Storage
| Powder | -20°C | 3 years |
|---|---|---|
| 4°C | 2 years | |
| In solvent | -80°C | 6 months |
| -20°C | 1 month |
Solvent & Solubility
DMSO : ≥ 100 mg/mL ( 423.24 mM )
Ethanol : 25 mg/mL ( 105.81 mM ; Need ultrasonic)
* "≥" means soluble, but saturation unknown.
Stock Solutions
| Concentration Solvent Mass | 1 mg | 5 mg | 10 mg |
|---|
| 1 mM | 4.2324 mL | 21.1622 mL | 42.3245 mL |
| 5 mM | 0.8465 mL | 4.2324 mL | 8.4649 mL |
| 10 mM | 0.4232 mL | 2.1162 mL | 4.2324 mL |
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1.
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2.
Add each solvent one by one: 10% DMSO >> 90% (20% SBE-β-CD in saline)
Solubility: 2.5 mg/mL (10.58 mM); Suspended solution; Need ultrasonic
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3.
Add each solvent one by one: 10% DMSO >> 90% corn oil
Solubility: ≥ 2.5 mg/mL (10.58 mM); Clear solution
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4.
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5.
Add each solvent one by one: 10% EtOH >> 90% (20% SBE-β-CD in saline)
Solubility: 2.5 mg/mL (10.58 mM); Suspended solution; Need ultrasonic
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6.
Add each solvent one by one: 10% EtOH >> 90% corn oil
Solubility: ≥ 2.5 mg/mL (10.58 mM); Clear solution
References
- [1] Okuyama T, et al. Structural and mechanistic insights into nuclear transport and delivery of the critical pluripotency factor Oct4 to DNA. J Biomol Struct Dyn. 2017 Feb 6:1-50
- [2] Li W, et al. Identification of Oct4-activating compounds that enhance reprogramming efficiency. Proc Natl Acad Sci U S A. 2012 Dec 18;109(51):20853-8.