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[CAS NO. 60857-08-1] Prostratin
| Ships within | Stock | Price | Qty | Total |
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| Catalog: | HY-107421 |
| Brand: | MCE |
| CAS: | 60857-08-1 |
Overview
| MDL | - |
|---|---|
| Molecular Weight | 390.47 |
| Molecular Formula | C22H30O6 |
| SMILES | CC(O[C@@]12[C@@]([C@@]3([H])[C@]([C@@H](C2)C)([C@@]4([H])[C@](CC(CO)=C3)(C(C(C)=C4)=O)O)O)([H])C1(C)C)=O |
Summary
IC50 & Target
|
PKC 12.5 nM (Ki) |
HIV-1
|
In Vitro
Prostratin inhibits [
3
H]PDBu binding to the CEM cells with a K
i
of 210 nM
[1]
.
Prostratin (125-1000 nM) dose-dependently inhibits the growth of acute myeloid leukemia (AML) cell lines (HL-60, NB4, and U937 cells). Prostratin (125-100 nM) induces G1 arrest of AML cells and affects the cell-cycle-related molecules (pRb phosphorylation, CDKs, and p21) in HL-60 cells. Prostratin also causes differentiation in AML cell lines via activation of PKC. Furthermore, PKC-dependent activation of the MEK/ERK/MAP signaling pathway requires differentiation induced by Prostratin
[2]
.
Prostratin induces HIV-1 transcription activation requiring active form of PKD3. Prostratin also activates PKD3 via PKCε of novel PKC subfamily
[3]
.
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Cell Viability Assay [2]
| Cell Line: | HL-60, NB4 and U937 cells |
| Concentration: | 125 nM, 250 nM, 500 nM, 1000 nM |
| Incubation Time: | 24 hours, 48 hours, 72 hours |
| Result: | Dose-dependently inhibited the growth of acute myeloid leukemia (AML) cell lines. |
Cell Cycle Analysis [2]
| Cell Line: | HL-60, NB4 and U937 cells |
| Concentration: | 125 nM, 250 nM, 500 nM, 1000 nM |
| Incubation Time: | 24 hours |
| Result: | Induced a G0/G1 phase accumulation in a concentration-dependent manner. |
Western Blot Analysis [2]
| Cell Line: | HL-60 cells |
| Concentration: | 125 nM, 250 nM, 500 nM, 1000 nM |
| Incubation Time: | 24 hours |
| Result: | Affected the cell-cycle-related molecules (pRb phosphorylation, CDKs, and p21) in HL-60 cells. |
Appearance
Solid
Shipping
Room temperature in continental US; may vary elsewhere.
Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
References
- [1] Gustafson KR, et al. A nonpromoting phorbol from the samoan medicinal plant Homalanthus nutans inhibits cell killing by HIV-1. J Med Chem. 1992 May 29;35(11):1978-86.
- [2] Shen X, et al. The protein kinase C agonist prostratin induces differentiation of human myeloid leukemia cells and enhances cellular differentiation by chemotherapeutic agents. Cancer Lett. 2015 Jan 28;356(2 Pt B):686-96.
- [3] Wang H, et al. Protein kinase D3 is essential for prostratin-activated transcription of integrated HIV-1 provirus promoter via NF-κB signaling pathway. Biomed Res Int. 2014;2014:968027.
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