[CAS NO. 71208-06-5] Lewis X trisaccharide
PRODUCTS SPECIFICATIONS [71208-06-5]
DESCRIPTION [71208-06-5]
Overview
| MDL | - |
|---|---|
| Molecular Weight | 529.49 |
| Molecular Formula | C20H35NO15 |
| SMILES | OC[C@H]([C@@H]([C@@H]([C@H]1O)O)O)O[C@H]1O[C@H]([C@H](O)CO)[C@@H]([C@H](C=O)NC(C)=O)O[C@H]2[C@H]([C@@H]([C@@H]([C@@H](O2)C)O)O)O |
Summary
Lewis X trisaccharide (Lewis X, Le x ) is a potent T H 2 regulator, antagonizes LPS-induced IL-12 immune expression. Lewis X trisaccharide is a human histo-blood group antigen, plays an key role in cell-cell adhesion, and servers as a tumor marker. Lewis X trisaccharide is highly expressed in the outer membrane of the parasite , can be used for the immunology research of schistosomiasis [1] [2] [3] .
In Vitro
Lewis X trisaccharide-BSA (25 μg/mL; 30 min; before LPS stimulation of 10 ng/mL for 4 h) IL-12p40 and suppresses IL-12p70 protein expression induced by
Lipopolysaccharide
(LPS, HY-D1056)
[1]
.
Lewis X trisaccharide (2 μM; 30 min; before LPS stimulation of 10 ng/mL for 2 h) decreases nuclear NF-κB concentration in mice bone marrow derived dendritic cells (BDDCs)
[1]
.
Lewis X trisaccharide-BSA (25 μg/mL; 48 h) or Lewis X trisaccharide (5 μg/mL; 48 h) plus ovalbumin (OVA, 25 μg/mL) increases cytokines (IL-4, IL-13, and INF-γ) level in mice splenocytes
[1]
.
Lewis X trisaccharide-containing glycoconjugates stimulates B cells to proliferate and to produce factors that down-regulates the TH
1
immune response and up-regulates the T
H
2 immune response
[2]
.
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
In Vivo
Lewis X trisaccharide ( 5 μg, combinded with 50 μg ovalbumin; s.c.; once a week for 2 weeks) regulates IgE/T
H
2 responses, and selectively increases the IgE and IgG1 responses in C3H mice, independent of the LPS-TLR4 signaling
[1]
.
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
| Animal Model: | Mice (BALB/c, IL-12 deficient on a BALB/c background, TLR4-defective C3H/hej, or TLR4-wild type C3H/HeOuj mice) (6-8 weeks old) [1] |
| Dosage: | 5 μg, combinded with 50 μg ovalbumin |
| Administration: | Subcutaneous injection; once a week for 2 weeks |
| Result: |
In C3H mice, coupled with BSA (Le
X
-BSA) and elicited higher levels of specific IgE and IgG1, but not IgG2a, which were associated with increased levels of splenic T
H
2 cytokines when compared with those seen in BSA-sensitized mice.
In BALB/c mice, induced by ovalbumin, significantly increased levels of specific IgE, resulted IgG2a antibodies concomitant with reduced levels of serum IL-12p70. In IL-12-deficient BALB/c mice, attenuated the downward trend of the above indicators. |
Source
human
Shipping
Room temperature in continental US; may vary elsewhere.
Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
References
- [1] Hsu SC, et al. Antigen coupled with Lewis-x trisaccharides elicits potent immune responses in mice. J Allergy Clin Immunol. 2007 Jun;119(6):1522-8.
- [2] van Roon AM, et al. Structure of an anti-Lewis X Fab fragment in complex with its Lewis X antigen. Structure. 2004 Jul;12(7):1227-36.
- [3] Topin J, et al. The Hidden Conformation of Lewis x, a Human Histo-Blood Group Antigen, Is a Determinant for Recognition by Pathogen Lectins. ACS Chem Biol. 2016 Jul 15;11(7):2011-20.
Synonyms