[CAS NO. 71555-25-4] NSC319726
PRODUCTS SPECIFICATIONS [71555-25-4]
DESCRIPTION [71555-25-4]
Overview
| MDL | MFCD01665446 |
|---|---|
| Molecular Weight | 234.32 |
| Molecular Formula | C11H14N4S |
| SMILES | C/C(C1=NC=CC=C1)=N\NC(N2CCC2)=S |
1 Publications Citing Use of MCE
Summary
NSC319726 (ZMC1) is a mutant p53R175 reactivator; inhibits growth of fibroblasts expressing the p53R175 mutation (IC50 = 8 nM); shows no inhibition for p53 wild-type cells.
IC50 & Target
IC50: 8 nM (mutant p53R175 reactivator) [1] .
In Vitro
For NSC319726, the effect was even greater as the IC50 for the 175 mutant was 8 nM while the IC50 of the WT was not reached. NSC319726 did not induce WT p53 protein levels or transcriptional activity as common cytotoxic agents such as etoposide do in vitro. NSC319726 exhibited a much higher sensitivity for the MEF-p53R172H/R172H cell line as compared to the p53+/+ and p53-/- controls. NSC319726 treatment of a MEF cell line derived from p53R172H/R172H mice resulted in a loss of PAB240 immunoflouresence staining.
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
In Vivo
At a dose of 1mg/kg, tumor growth of the H460 (p53+/+) and MDAMB468 (p53R273W) xenografts was not inhibited relative to the vehicle control whereas tumor growth was significantly inhibited in the TOV112D (p53R175H) xenografts. When we lowered the dose ten-fold to 0.1 mg/kg in the TOV112D mice, we observed only a small difference in tumor growth inhibition demonstrating both a dosage effect of the drug and a larger therapeutic window.Taken together, these findings provide in vivo evidence for allele specific p53 mutant reactivation.
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Appearance
Solid
Shipping
Room temperature in continental US; may vary elsewhere.
Storage
| Powder | -20°C | 3 years |
|---|---|---|
| 4°C | 2 years | |
| In solvent | -80°C | 6 months |
| -20°C | 1 month |
Solvent & Solubility
DMSO : 31.25 mg/mL ( 133.36 mM ; Need ultrasonic)
Stock Solutions
| Concentration Solvent Mass | 1 mg | 5 mg | 10 mg |
|---|
| 1 mM | 4.2677 mL | 21.3383 mL | 42.6767 mL |
| 5 mM | 0.8535 mL | 4.2677 mL | 8.5353 mL |
| 10 mM | 0.4268 mL | 2.1338 mL | 4.2677 mL |
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Add each solvent one by one: 10% DMSO >> 90% corn oil
Solubility: ≥ 2.08 mg/mL (8.88 mM); Clear solution
References
Synonyms