[CAS NO. 7365-45-9] HEPES
PRODUCTS SPECIFICATIONS [7365-45-9]
DESCRIPTION [7365-45-9]
Overview
| MDL | MFCD00006158 |
|---|---|
| Molecular Weight | 238.30 |
| Molecular Formula | C8H18N2O4S |
| SMILES | O=S(CCN1CCN(CCO)CC1)(O)=O |
1 Publications Citing Use of MCE
Summary
HEPES, a nonvolatile zwitterionic chemical buffering agent, is broadly applied in cell culture . HEPES is effective at pH 6.8 to 8.2. HEPES is also a potent inducer of lysosome biogenesis [1] [2] [3] .
In Vitro
HEPES maintains superhydrophilicity of titanium for at least 3 months and resulted in a continuous retention of bioactivity and osteoconductivity
[1]
.
HEPES drives lysosome biogenesis, affects MiT/TFE cytoplasmic-nuclear distribution, disrupts global cellular transcriptional profiles, resulting the activation of a MiT/TFE-dependent lysosomal-autophagic gene network in cultured RAW264.7 cells
[3]
.
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Appearance
Solid
Shipping
Room temperature in continental US; may vary elsewhere.
Storage
| Powder | -20°C | 3 years |
|---|---|---|
| 4°C | 2 years | |
| In solvent | -80°C | 6 months |
| -20°C | 1 month |
Solvent & Solubility
H 2 O : 250 mg/mL ( 1049.10 mM ; Need ultrasonic)
Stock Solutions
| Concentration Solvent Mass | 1 mg | 5 mg | 10 mg |
|---|
| 1 mM | 4.1964 mL | 20.9820 mL | 41.9639 mL |
| 5 mM | 0.8393 mL | 4.1964 mL | 8.3928 mL |
| 10 mM | 0.4196 mL | 2.0982 mL | 4.1964 mL |
-
1.
Add each solvent one by one: PBS
Solubility: 100 mg/mL (419.64 mM); Clear solution; Need ultrasonic
Synonyms