[CAS NO. 7385-67-3] Nile Red
PRODUCTS SPECIFICATIONS [7385-67-3]
DESCRIPTION [7385-67-3]
Overview
| MDL | MFCD00011639 |
|---|---|
| Molecular Weight | 318.37 |
| Molecular Formula | C20H18N2O2 |
| SMILES | O=C1C2=CC=CC=C2C3=NC4=CC=C(N(CC)CC)C=C4OC3=C1 |
23 Publications Citing Use of MCE
- [1]Nano Today. 47 (2022) 101675
- [2]Nat Commun. 2022 Oct 5;13(1):5871.
- [3]Nat Commun. 2020 Jan 13;11(1):240.
- [4]Nucleic Acids Res. 2022 Jun 24;50(12):6953-6967.
- [5]EMBO J. 2022 Jul 4;e110439.
- [6]Theranostics. 2022 May 26;12(10):4513-4535.
- [7]Theranostics. 2021 Jan 1;11(5):2149-2169.
- [8]Clin Transl Med. 2023 Jan;13(1):e1158.
- [9]J Control Release. 2021 Apr 10;332:194-209.
- [10]Pharmacol Res. 2023 Jan 16;188:106666.
- [11]Cell Prolif. 2021 Sep 25;e13134.
- [12]Int J Mol Sci. 2021 May 31;22(11):5951.
- [13]Food Funct. 2021 Mar 7;12(5):2171-2188.
- [14]J Transl Med. 2022 May 14;20(1):222.
- [15]Antiviral Res. 2023 Jan 13;211:105542.
- [16]Endocrinology. 2023 Jan 2;bqac215.
- [17]Life Sci. 2018 Jul 1;204:55-64.
- [18]Brain Res Bull. 31 October 2022.
- [19]Eur J Pharmacol. 2022 Nov 24;175428.
- [20]RSC Adv. 2021 Nov 2;11(56):35331-35341.
- [21]ACS Omega. 2022.
- [22]Theriogenology. 2023 Jan 13.
- [23]Research Square Print. October 20th, 2022.
Summary
Nile red (Nile blue oxazone) is a lipophilic stain. Nile red has environment-sensitive fluorescence. Nile red is intensely fluorescent in a lipid-rich environment while it has minimal fluorescence in aqueous media. Nile red is an excellent vital stain for the detection of intracellular lipid droplets by fluorescence microscopy and flow cytof uorometry. Nile red stains intracellular lipid droplets red. The fluorescence wavelength is 559/635 nm [1] .
In Vitro
1. Preparation of Phalloidin-TRITC working solution
1.1Preparation of the stock solution
Dissolve Phalloidin-TRITC in Methanol to obtain 10 mM of stock solution.
Note: It is recommended to store the stock solution at -20℃ or -80℃ away from light and avoid repetitive freeze-thaw cycles.
1.2 Preparation of Phalloidin-TRITC working solution
Dilute the stock solution in serum-free cell culture medium to obtain 1-10 μM of working solution.
Note: Please adjust the concentration of Phalloidin-TRITC working solution according to the actual situation.
2. Cell staining
2.1 Suspension cells (6-well plate)
a.Centrifuge at 1000 g at 4℃ for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.
b.Add 1 mL of working solution, and then incubate at room temperature for 30-60 minutes.
c.Centrifuge at 400 g at 4℃ for 3-4 minutes and then discard the supernatant.
d.Wash twice with PBS, 5 minutes each time.
e.Resuspend cells with serum-free cell culture medium or PBS. Observation by fluorescence microscopy or flow cytometry.
2.2 Adherent cells
a.Culture adherent cells on sterile coverslips.
b.Remove the coverslip from the medium and aspirate excess medium.
c.Add 100 μL of working solution, gently shake it to completely cover the cells,and then incubate at room temperature for 30-60 minutes.
d.Wash twice with medium, 5 minutes each time. Observation by fluorescence microscopy.
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
In Vivo
When Nile red-stained Caenorhabditis elegans is viewed for green fluorescence, discrete lipid bodies can be observed throughout the intestine and other tissues either in clusters or evenly dispersed, depending on the animal's genotype or experimental treatment [3] .
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Appearance
Solid
Shipping
Room temperature in continental US; may vary elsewhere.
Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
DMSO : ≥ 50 mg/mL ( 157.05 mM )
H 2 O : < 0.1 mg/mL (insoluble)
* "≥" means soluble, but saturation unknown.
Stock Solutions
| Concentration Solvent Mass | 1 mg | 5 mg | 10 mg |
|---|
| 1 mM | 3.1410 mL | 15.7050 mL | 31.4100 mL |
| 5 mM | 0.6282 mL | 3.1410 mL | 6.2820 mL |
| 10 mM | 0.3141 mL | 1.5705 mL | 3.1410 mL |
References
- [1] Greenspan P, et al. Nile red: a selective fluorescent stain for intracellular lipid droplets. J Cell Biol. 1985 Mar;100(3):965-73.
- [2] Greenspan P, et al. Nile red: a selective fluorescent stain for intracellular lipid droplets. J Cell Biol. 1985 Mar;100(3):965-73.
- [3] Gibrán S Alemán-Nava, et al. How to use Nile Red, a selective fluorescent stain for microalgal neutral lipids. J Microbiol Methods. 2016 Sep;128:74-79.
- [4] Wilber Escorcia, et al. Quantification of Lipid Abundance and Evaluation of Lipid Distribution in Caenorhabditis elegans by Nile Red and Oil Red O Staining. J Vis Exp. 2018 Mar 5;(133):57352.
- [5] Elizabeth C Pino, et al. Biochemical and high throughput microscopic assessment of fat mass in Caenorhabditis elegans. J Vis Exp. 2013 Mar 30;(73):50180.
Synonyms