[CAS NO. 7423-31-6] Stains-All

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PRODUCTS SPECIFICATIONS [7423-31-6]

Catalog

HY-D0987

Brand

MCE

CAS

7423-31-6

DESCRIPTION [7423-31-6]

Overview

MDL	MFCD00078788
Molecular Weight	559.58
Molecular Formula	C30H27BrN2S2
SMILES	CC(/C=C1SC2=CC=C3C=CC=CC3=C2N\1CC)=C\C4=[N+](CC)C5=C6C=CC=CC6=CC=C5S4.[Br-]

For research use only. We do not sell to patients.

Summary

Stains-All, a cationic carbocyanine dye, is a convenient probe to study the structural features of the individual calcium-binding sites of calmodulin (CaM) and related calcium-binding proteins (CaBP) ^[1] ^[2] .

In Vitro

Almost all of the proteins found in skeletal muscle extracts, including the Ca ²⁺ +Mg ²⁺ -ATPase and the 53,000-Da glycoprotein of the sarcoplasmic reticulum, are stained red or pink with Stains-all. Calsequestrin, the 1 60,000-Da glycoprotein, and 170,000-Da protein are stained blue with Stains-all. The ratio of Stains-all staining (measured at 615 nm) to that of Coomassie blue staining (measured at 575 nm) is 1.3 for calsequestrin, 2.0 for calmodulin, 1.4 for troponin C, and 2.2 for S-100. Therefore, in addition to differentially staining these Ca ²⁺ -binding proteins blue, Stains-all is a more sensitive stain for these Ca ²⁺ -binding proteins than is Coomassie blue ^[3] .

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Appearance

Solid

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture and light

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility

In Vitro:

DMSO : 8.33 mg/mL ( 14.89 mM ; ultrasonic and warming and heat to 80°C)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.7871 mL	8.9353 mL	17.8705 mL
5 mM	0.3574 mL	1.7871 mL	3.5741 mL
10 mM	0.1787 mL	0.8935 mL	1.7871 mL

* Please refer to the solubility information to select the appropriate solvent.

References

Synonyms

Naphtho[1,2-d]thiazolium, 1-ethyl-2-[3-(1-ethylnaphtho[1,2-d]thiazol-2(1H)-ylidene)-2-methyl-1-propen-1-yl]-, bromide (1:1)

Naphtho[1,2-d]thiazolium, 1-ethyl-2-[3-(1-ethylnaphtho[1,2-d]thiazolin-2-ylidene)-2-methylpropenyl]-, bromide

Naphtho[1,2-d]thiazolium, 1-ethyl-2-[3-(1-ethylnaphtho[1,2-d]thiazol-2(1H)-ylidene)-2-methyl-1-propenyl]-, bromide

1-Ethyl-2-[3-(1-ethylnaphtho[1,2-d]thiazolin-2-ylidene)-2-methylpropenyl]naphtho[1,2-d]thiazolium bromide

4,5,4′,5′-Dibenzothiacarbocyanine bromide, 3,3′-diethyl-9-methyl-

3,3′-Diethyl-9-methyl-4,5,4′,5′-dibenzothiacarbocyanine bromide

Stains-all

Carbocyanin DBTC

DBTC

4,5,4′,5′-Dibenzo-3,3′-diethyl-9-methylthiacarbocyanine bromide

3,3′-Diethyl-9-methyl-4,5,4′,5′-naphthothiacarbocyanine bromide

1-Ethyl-2-(3-(1-ethylnaphtho[1,2-d]thiazol-2(1H)-ylidene)-2-methylprop-1-en-1-yl)naphtho[1,2-d]thiazol-1-ium bromide