[CAS NO. 803647-40-7] NSC59984
PRODUCTS SPECIFICATIONS [803647-40-7]
DESCRIPTION [803647-40-7]
Overview
| MDL | MFCD29905462 |
|---|---|
| Molecular Weight | 265.27 |
| Molecular Formula | C12H15N3O4 |
| SMILES | O=C(N1CCN(C)CC1)/C=C/C2=CC=C([N+]([O-])=O)O2 |
1 Publications Citing Use of MCE
Summary
In Vitro
NSC59984 specifically restores p53 pathway signaling in mutant p53-expressing human colorectal cancer cells. NSC59984 induces cell death in tumor cells but not normal cells with little or no genotoxicity. NSC59984 induces mutant p53 protein degradation through MDM2-mediated ubiquitination in cancer cells. NSC59984 restores p53 pathway signaling through activation of p73. NSC59984 induces p73-dependent cell apoptosis in cancer. The EC50 of NSC59984 in most cancer cells is significantly lower than those of normal cells, with EC50 of 8.38 μM for p53-null HCT116 cells [1] .
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
In Vivo
NSC59984 synergizes with CPT11 to induce cell death in mutant p53-expressing colorectal cancer cells and inhibits mutant p53-associated colon tumor xenograft growth in a p73-dependent manner [1] .
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Appearance
Solid
Shipping
Room temperature in continental US; may vary elsewhere.
Storage
| Powder | -20°C | 3 years |
|---|---|---|
| 4°C | 2 years | |
| In solvent | -80°C | 6 months |
| -20°C | 1 month |
Solvent & Solubility
DMSO : 33.33 mg/mL ( 125.65 mM ; Need ultrasonic)
Stock Solutions
| Concentration Solvent Mass | 1 mg | 5 mg | 10 mg |
|---|
| 1 mM | 3.7697 mL | 18.8487 mL | 37.6974 mL |
| 5 mM | 0.7539 mL | 3.7697 mL | 7.5395 mL |
| 10 mM | 0.3770 mL | 1.8849 mL | 3.7697 mL |
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Add each solvent one by one: 10% DMSO >> 90% (20% SBE-β-CD in saline)
Solubility: ≥ 2.5 mg/mL (9.42 mM); Clear solution
References
- [1] Zhang S, et al. Small-Molecule NSC59984 Restores p53 Pathway Signaling and Antitumor Effects against Colorectal Cancer via p73 Activation and Degradation of Mutant p53. Cancer Res. 2015 Sep 15;75(18):3842-52.
- [2] Zhang S, et al. Small-Molecule NSC59984 Restores p53 Pathway Signaling and Antitumor Effects againstColorectal Cancer via p73 Activation and Degradation of Mutant p53. Cancer Res. 2015 Sep 15;75(18):3842-52.